| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAB1B (7-201) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAB1B |
| Gene ID: |
81876 |
| Uniprot ID: |
RAB1B_HUMAN |
| Immunogen Region: |
7-201 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 7-201 of human RAB1B (NP_112243.1). |
| Immunogen Sequence: |
YLFKLLLIGDSGVGKSCLLL RFADDTYTESYISTIGVDFK IRTIELDGKTIKLQIWDTAG QERFRTITSSYYRGAHGIIV VYDVTDQESYANVKQWLQEI DRYASENVNKLLVGNKSDLT TKKVVDNTTAKEFADSLGIP FLETSAKNATNVEQAFMTMA AEIKKRMGPGAASGGERPNL KIDSTPVKPAGGGCC |
| Post Translational Modifications | Prenylated.by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). (Microbial infection) AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. It is later de-AMPylated by L.pneumophila SidD, releasing RAB1B from bacterial phagosomes. (Microbial infection) Phosphocholinated at Ser-76 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment. |
| Protein Name | Ras-Related Protein Rab-1b |
| Database Links | Reactome: R-HSA-162658Reactome: R-HSA-204005Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | CytoplasmMembraneLipid-AnchorCytoplasmic SidePreautophagosomal Structure MembranePerinuclear RegionTargeted By Rep1 To Membranes Of Specific Subcellular Compartments Including Endoplasmic ReticulumGolgi ApparatusAnd Intermediate Vesicles Between These Two CompartmentsIn The Gdp-FormColocalizes With Gdi In The CytoplasmCo-Localizes With Mtmr6 To The Endoplasmic Reticulum-Golgi Intermediate Compartment And To The Peri-Golgi Region |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-1b antibodyAnti-RAB1B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
