• Anti-Rab1b antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-Rab1bAb using hCEC cells transduced with GFP-Rab1b; cells were fixed with methanol and anti-Rab5a at 1:250
  • Immunohistochemistry of human breast carcinoma using anti-Rab1b antibody and FFPE tissue after heat-induced antigen retrieval; Anti-Rab1b antibody at 1:750/DAB detection
  • Immunohistochemistry of human pancreas using anti-Rab1b antibody and FFPE tissue after heat-induced antigen retrieval; Anti-Rab1b antibody at 1:750/DAB detection
  • Immunohistochemistry of rat lung using anti-Rab1b antibody and FFPE tissue after heat-induced antigen retrieval; Anti-Rab1b antibody at 1:750/DAB detection

Anti-RAB1B antibody (110aa C-Term) (STJ140140)

SKU:
STJ140140

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF
Reactivity: Human/Rat/Mouse/Monkey/Canine
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-RAB1, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:250-1:5000
IF 1:100-1:500
IHC-F 1:250-1:1000
IHC-P 1:250-1:1000
Storage Instruction: Store at-20 C for long-term storage. Store at 2-8 C for up to one month.
Gene Symbol: RAB1B
Gene ID: 81876
Uniprot ID: RAB1B_HUMAN
Immunogen Region: 110aa C-Term
Accession Number: ENSG00000174903
Specificity: Detects endogenous levels of Rab1b protein by Western blot in whole cell lysates and transfected cells with GFP-Rab1b cds.
Immunogen: Purified recombinant peptide derived from within residues 110 aa to the C-terminus of Rab1b produced in E. coli.
Immunogen Sequence: QEIDRYASENVNKLLVGNKS DLTTKKVVDNTTAKEFADSL GVPFLETSAKNATNVEQAFM TMAAEIKKRMGPGAASGGER PNLKIDSTPVKPASGGCC
Post Translational Modifications Prenylated.by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1). (Microbial infection) AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active. It is later de-AMPylated by L.pneumophila SidD, releasing RAB1B from bacterial phagosomes. (Microbial infection) Phosphocholinated at Ser-76 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Plays a role in the initial events of the autophagic vacuole development which take place at specialized regions of the endoplasmic reticulum. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments. Required to modulate the compacted morphology of the Golgi. Promotes the recruitment of lipid phosphatase MTMR6 to the endoplasmic reticulum-Golgi intermediate compartment.
Protein Name Ras-Related Protein Rab-1b
Database Links Reactome: R-HSA-162658
Reactome: R-HSA-204005
Reactome: R-HSA-6807878
Reactome: R-HSA-6811434
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cytoplasm
Membrane
Lipid-Anchor
Cytoplasmic Side
Preautophagosomal Structure Membrane
Perinuclear Region
Targeted By Rep1 To Membranes Of Specific Subcellular Compartments Including Endoplasmic Reticulum
Golgi Apparatus
And Intermediate Vesicles Between These Two Compartments
In The Gdp-Form
Colocalizes With Gdi In The Cytoplasm
Co-Localizes With Mtmr6 To The Endoplasmic Reticulum-Golgi Intermediate Compartment And To The Peri-Golgi Region
Alternative Antibody Names Anti-Ras-Related Protein Rab-1b antibody
Anti-RAB1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance