| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF/ELISA |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB1, member RAS oncogene family (110aa C-Term) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:1000IHC-F 1:250-1:1500IHC-P 1:250-1:1500IF 1:50-1:250ELISA NDMojallal M Zheng Y Hultin S et al. Nat Commun 2014 Aug 1:5:4557. PMID: 25080966 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
RAB1A |
| Gene ID: |
5861 |
| Uniprot ID: |
RAB1A_HUMAN |
| Immunogen Region: |
110aa C-Term |
| Accession Number: |
ENSG00000138069 |
| Specificity: |
Detects total Rab1 protein by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab1a and GFP-Rab1b cds. |
| Immunogen: |
Purified recombinant peptides derived from within residues 110 aa to the C-terminus of mouse Rab1a and Rab1b produced in E. coli. |
| Post Translational Modifications | Phosphorylated by CDK1 kinase during mitosis. (Microbial infection) Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Required to modulate the compacted morphology of the Golgi. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport. |
| Protein Name | Ras-Related Protein Rab-1aYpt1-Related Protein |
| Database Links | Reactome: R-HSA-162658Reactome: R-HSA-204005Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Golgi ApparatusEndoplasmic ReticulumEarly EndosomeCytoplasmCytosolMembraneMelanosomeAlternates Between Membrane-Associated And Cytosolic Forms |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-1a antibodyAnti-Ypt1-Related Protein antibodyAnti-RAB1A antibodyAnti-RAB1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
