Anti-RAB1A antibody (1-205) (STJ119490)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAB1A (1-205) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB1A |
| Gene ID | 5861 |
| Uniprot ID | RAB1A_HUMAN |
| Immunogen Region | 1-205 |
| Immunogen Sequence | MSSMNPEYDYLFKLLLIGDS GVGKSCLLLRFADDTYTESY ISTIGVDFKIRTIELDGKTI KLQIWDTAGQERFRTITSSY YRGAHGIIVVYDVTDQESFN NVKQWLQEIDRYASENVNKL LVGNKCDLTTKKVVDYTTAK EFADSLGIPFLETSAKNATN VEQSFMTMAAEIKKRMGPGA TAGGAEKSNVKIQSTPVKQS GGGCC |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-205 of human RAB1A (NP_004152.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by CDK1 kinase during mitosis. (Microbial infection) Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB. (Microbial infection) Glycosylated by S.typhimurium protein Ssek3: arginine GlcNAcylation prevents GTPase activity, thereby disrupting vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment. Ubiquitinated via 'Lys-11'-linked ubiquitination on Lys-49 and Lys-61.impairing the recruitment of guanosine diphosphate (GDP) dissociation inhibitor 1/GDI1. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Required to modulate the compacted morphology of the Golgi. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport. |
| Protein Name | Ras-Related Protein Rab-1aYpt1-Related Protein |
| Database Links | Reactome: R-HSA-162658Reactome: R-HSA-204005Reactome: R-HSA-6807878Reactome: R-HSA-6811434Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Golgi ApparatusEndoplasmic ReticulumEarly EndosomeCytoplasmCytosolMembraneMelanosomeAlternates Between Membrane-Associated And Cytosolic Forms |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-1a antibodyAnti-Ypt1-Related Protein antibodyAnti-RAB1A antibodyAnti-RAB1 antibody |
Information sourced from Uniprot.org