| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-RAB17 (1-212) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAB17 |
| Gene ID: |
64284 |
| Uniprot ID: |
RAB17_HUMAN |
| Immunogen Region: |
1-212 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human RAB17 (NP_071894.1). |
| Immunogen Sequence: |
MAQAHRTPQPRAAPSQPRVF KLVLLGSGSVGKSSLALRYV KNDFKSILPTVGCAFFTKVV DVGATSLKLEIWDTAGQEKY HSVCHLYFRGANAALLVYDI TRKDSFLKAQQWLKDLEEEL HPGEVLVMLVGNKTDLSQER EVTFQEGKEFADSQKLLFME TSAKLNHQVSEVFNTVAQEL LQRSDEEGQALRGDAAVALN KGPARQAKCCAH |
| Tissue Specificity | Expressed in melanocytes (at protein level). |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. Mainly observed in epithelial cells, transcytosis mediates for instance, the transcellular transport of immunoglobulins from the basolateral surface to the apical surface. Most probably controls membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis. Required for melanosome transport and release from melanocytes, it also regulates dendrite and dendritic spine development. May also play a role in cell migration. |
| Protein Name | Ras-Related Protein Rab-17 |
| Database Links | Reactome: R-HSA-8873719 |
| Cellular Localisation | Recycling Endosome MembraneLipid-AnchorCytoplasmic SideMelanosomeCell ProjectionDendriteMay Also Localize At The Basolateral And Apical Plasma MembraneIn NeuronsLocalizes To The Cell Body And Dendritic Shaft And Spine |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-17 antibodyAnti-RAB17 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/96554/379107/STJ117595_1__37100.1713146827.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PDCD10 knockout (KO) HeLa cells, using [KO Validated] PDCD10 Rabbit polyclonal antibody (STJ117595) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.")