Anti-RAB14 antibody (39-140) (STJ114625)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAB14 (39-140) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB14 |
| Gene ID | 51552 |
| Uniprot ID | RAB14_HUMAN |
| Immunogen Region | 39-140 |
| Immunogen Sequence | DCPHTIGVEFGTRIIEVSGQ KIKLQIWDTAGQERFRAVTR SYYRGAAGALMVYDITRRST YNHLSSWLTDARNLTNPNTV IILIGNKADLEAQRDVTYEE AK |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 39-140 of human RAB14 (NP_057406.2). |
Additional Info
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. Involved in membrane trafficking between the Golgi complex and endosomes during early embryonic development. Regulates the Golgi to endosome transport of FGFR-containing vesicles during early development, a key process for developing basement membrane and epiblast and primitive endoderm lineages during early postimplantation development. May act by modulating the kinesin KIF16B-cargo association to endosomes. Regulates, together with its guanine nucleotide exchange factor DENND6A, the specific endocytic transport of ADAM10, N-cadherin/CDH2 shedding and cell-cell adhesion. Mediates endosomal tethering and fusion through the interaction with RUFY1 and RAB4B. Interaction with RAB11FIP1 may function in the process of neurite formation. |
| Protein Name | Ras-Related Protein Rab-14 |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1660499Reactome: R-HSA-6798695Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Recycling EndosomeEarly Endosome MembraneLipid-AnchorCytoplasmic SideGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneCytoplasmic VesiclePhagosomeRecruited To Recycling Endosomes By Dennd6aRecruited To Phagosomes Containing SAureus Or MTuberculosisColocalizes With Rab11fip1 On Punctate Vesicles |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-14 antibodyAnti-RAB14 antibody |
Information sourced from Uniprot.org