Anti-RAB13 antibody (40-120 aa) (STJ191269)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 40-120
General Information
| Short Description | Rabbit polyclonal anti-Ras-related protein Rab-13 (40-120 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB13 |
| Gene ID | 5872 |
| Uniprot ID | RAB13_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 40-120 |
| Immunogen Region | 40-120 aa |
| Specificity | RAB13 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | (Microbial infection) Stearoylated By S.flexneri N-epsilon-fatty acyltransferase IcsB, thereby disrupting the host actin cytoskeleton. Ubiquitinated via 'Lys-11'-linked ubiquitination on Lys-46 and Lys-58.impairing the recruitment of guanosine diphosphate (GDP) dissociation inhibitor 1/GDI1. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB13 is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis. |
| Protein Name | Ras-Related Protein Rab-13Cell Growth-Inhibiting Gene 4 Protein |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideCytoplasmic Vesicle MembraneCell JunctionTight JunctionGolgi ApparatusTrans-Golgi Network MembraneRecycling Endosome MembraneCell ProjectionLamellipodiumTight Junctions Or Associated With Vesicles Scattered Throughout The Cytoplasm In Cells Lacking Tight JunctionsRelocalizes To The Leading Edge Of Lamellipodia In Migrating Endothelial Cells |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-13 antibodyAnti-Cell Growth-Inhibiting Gene 4 Protein antibodyAnti-RAB13 antibodyAnti-GIG4 antibody |
Information sourced from Uniprot.org