Anti-RAB11FIP3 antibody (569-618 aa) (STJ95309)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RAB11FIP3 at the amino acid range 569-618
General Information
| Short Description | Rabbit polyclonal anti-Rab11 family-interacting protein 3 (569-618 aa) for use in IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAB11FIP3 |
| Gene ID | 9727 |
| Uniprot ID | RFIP3_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RAB11FIP3 at the amino acid range 569-618 |
| Immunogen Region | 569-618 aa |
| Specificity | Rab11-FIP3 Polyclonal Antibody detects endogenous levels of Rab11-FIP3 protein. |
Additional Info
| Function | Downstream effector molecule for Rab11 GTPase which is involved in endocytic trafficking, cytokinesis and intracellular ciliogenesis by participating in membrane delivery. Recruited by Rab11 to endosomes where it links Rab11 to dynein motor complex. The functional Rab11-RAB11FIP3-dynein complex regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endocytic recycling compartment (ERC) during interphase of cell cycle. Facilitates the interaction between dynein and dynactin and activates dynein processivity. Binding with ASAP1 is needed to regulate the pericentrosomal localization of recycling endosomes. The Rab11-RAB11FIP3 complex is also implicated in the transport during telophase of vesicles derived from recycling endosomes to the cleavage furrow via centrosome-anchored microtubules, where the vesicles function to deliver membrane during late cytokinesis and abscission. The recruitment of Rab11-RAB11FIP3-containing endosomes to the cleavage furrow and tethering to the midbody is co-mediated by RAB11FIP3 interaction with ARF6-exocyst and RACGAP1-MKLP1 tethering complexes. Also involved in the Rab11-Rabin8-Rab8 ciliogenesis cascade by facilitating the orderly assembly of a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which directs preciliary vesicle trafficking to mother centriole and ciliogenesis initiation. Also promotes the activity of Rab11 and ASAP1 in the ARF4-dependent Golgi-to-cilia transport of the sensory receptor rhodopsin. Competes with WDR44 for binding to Rab11, which controls intracellular ciliogenesis pathway. May play a role in breast cancer cell motility by regulating actin cytoskeleton. |
| Protein Name | Rab11 Family-Interacting Protein 3Fip3Fip3-Rab11Rab11-Fip3Arfophilin-1Ef Hands-Containing Rab-Interacting ProteinEferinMu-Mb-17.148 |
| Database Links | Reactome: R-HSA-5620916 |
| Cellular Localisation | Endosome MembraneRecycling Endosome MembranePeripheral Membrane ProteinCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCleavage FurrowMidbodyGolgi Apparatus MembraneGolgi ApparatusTrans-Golgi Network MembraneDuring InterphaseLocalized In Vesicles Continuously Moving From Peripheral Sorting Endosomes In The Cell Towards The Pericentrosomal Endosomal Recycling Compartment (Erc)In Early Mitosis Remains Diffuse And Distributed Through The CellThe Onset Of Anaphase Sequesters These Vesicles To The Centrosomes At The Opposite Poles Of The CellDuring Telophase These Vesicles Move From The CentrosomesTo The FurrowAnd Then To The Midbody To Aid In AbscissionInteraction With Rab11 Mediates Localization To EndosomesInteraction With Arf6 Mediates Localization To The MidbodyLocalized To The Golgi And Tgn When Interacting With Rho In PhotoreceptorsLocalized To Rhodopsin Transport Carriers When Interacting With Rab11a And Asap1 In Photoreceptors |
| Alternative Antibody Names | Anti-Rab11 Family-Interacting Protein 3 antibodyAnti-Fip3 antibodyAnti-Fip3-Rab11 antibodyAnti-Rab11-Fip3 antibodyAnti-Arfophilin-1 antibodyAnti-Ef Hands-Containing Rab-Interacting Protein antibodyAnti-Eferin antibodyAnti-Mu-Mb-17.148 antibodyAnti-RAB11FIP3 antibodyAnti-ARFO1 antibodyAnti-KIAA0665 antibody |
Information sourced from Uniprot.org