| Host: |
Goat |
| Applications: |
WB/IHC-F/IHC-P/IF |
| Reactivity: |
Human/Rat/Mouse/Monkey/Canine |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB11b, member RAS oncogene family (120aa C-Term) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS, 20% Glycerol and 0.05% Sodium Azide. |
| Purification: |
This antibody is epitope-affinity purified from goat antiserum. |
| Concentration: |
3 mg/ml |
| Dilution Range: |
WB 1:250-1:2000IF 1:50-1:250IHC-P 1:100-1:400IHC-F 1:100-1:400Cardoso MHS PhD Thesis NOVA University of Lisbon Portugal 2018 |
| Storage Instruction: |
For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours. |
| Gene Symbol: |
RAB11B |
| Gene ID: |
9230 |
| Uniprot ID: |
RB11B_HUMAN |
| Immunogen Region: |
120aa C-Term |
| Accession Number: |
ENSG00000185236 |
| Specificity: |
Detects a band of 25 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab11b cds. This Ab is specific for Rab11b, it does not recognize Rab11a. |
| Immunogen: |
Purified recombinant peptide derived from within residues 120 aa to the C-terminus of mouse Rab11b produced in E. coli. |
| Post Translational Modifications | Citrullinated by PADI4. (Microbial infection) Glycosylated on arginine residues by S.typhimurium protein Ssek3. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. The small Rab GTPase RAB11B plays a role in endocytic recycling, regulating apical recycling of several transmembrane proteins including cystic fibrosis transmembrane conductance regulator/CFTR, epithelial sodium channel/ENaC, potassium voltage-gated channel, and voltage-dependent L-type calcium channel. May also regulate constitutive and regulated secretion, like insulin granule exocytosis. Required for melanosome transport and release from melanocytes. Also regulates V-ATPase intracellular transport in response to extracellular acidosis. Promotes Rabin8/RAB3IP preciliary vesicular trafficking to mother centriole by forming a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, thereby regulating ciliogenesis initiation (Probable). On the contrary, upon LPAR1 receptor signaling pathway activation, interaction with phosphorylated WDR44 prevents Rab11-RAB3IP-RAB11FIP3 complex formation and cilia growth (Probable). |
| Protein Name | Ras-Related Protein Rab-11bGtp-Binding Protein Ypt3 |
| Database Links | Reactome: R-HSA-8854214Reactome: R-HSA-8873719 |
| Cellular Localisation | Recycling Endosome MembraneLipid-AnchorCytoplasmic SideCytoplasmic VesicleSecretory VesicleSynaptic Vesicle MembranePhagosome MembraneRecruited To Phagosomes Containing SAureus |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-11b antibodyAnti-Gtp-Binding Protein Ypt3 antibodyAnti-RAB11B antibodyAnti-YPT3 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
