• Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g PYGL antibody (STJ28793). Western blot was performed from the immunoprecipitate using PYGL antibody (STJ28793) at a dilution of 1:1000.
  • Immunofluorescence analysis of HepG2 cells using PYGL Rabbit polyclonal antibody (STJ28793) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PYGL Rabbit polyclonal antibody (STJ28793) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of PYGL in paraffin-embedded human liver using PYGL Rabbit polyclonal antibody (STJ28793) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PYGL Rabbit polyclonal antibody (STJ28793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PYGL antibody (690-847) (STJ28793)

SKU:
STJ28793

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PYGL (690-847) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYGL
Gene ID: 5836
Uniprot ID: PYGL_HUMAN
Immunogen Region: 690-847
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 690-847 of human PYGL (NP_002854.3).
Immunogen Sequence: IGTMDGANVEMAEEAGEENL FIFGMRIDDVAALDKKGYEA KEYYEALPELKLVIDQIDNG FFSPKQPDLFKDIINMLFYH DRFKVFADYEAYVKCQDKVS QLYMNPKAWNTMVLKNIAAS GKFSSDRTIKEYAQNIWNVE PSDLKISLSNESNKVNGN
Post Translational Modifications Acetylation, which is up-regulated by glucose and insulin and down-regulated by glucagon, inhibits the glycogen phosphorylase activity by promoting PPP1R3B-mediated recruitment of phosphatase PP1 and Ser-15 dephosphorylation. Phosphorylation at Ser-15 converts inactive phosphorylase b into active phosphorylase a. Dephosphorylation of Ser-15 by phosphatase PP1 inactivates the enzyme.
Function Allosteric enzyme that catalyzes the rate-limiting step in glycogen catabolism, the phosphorolytic cleavage of glycogen to produce glucose-1-phosphate, and plays a central role in maintaining cellular and organismal glucose homeostasis.
Protein Name Glycogen Phosphorylase - Liver Form
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-70221
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Glycogen Phosphorylase - Liver Form antibody
Anti-PYGL antibody

Information sourced from Uniprot.org

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