• Western blot analysis of HepG2, using PYCR1 Rabbit polyclonal antibody (STJ115309) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PYCR1 antibody (1-319) (STJ115309)

SKU:
STJ115309

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PYCR1 (1-319) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYCR1
Gene ID: 5831
Uniprot ID: P5CR1_HUMAN
Immunogen Region: 1-319
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-319 of human PYCR1 (NP_008838.2).
Immunogen Sequence: MSVGFIGAGQLAFALAKGFT AAGVLAAHKIMASSPDMDLA TVSALRKMGVKLTPHNKETV QHSDVLFLAVKPHIIPFILD EIGADIEDRHIVVSCAAGVT ISSIEKKLSAFRPAPRVIRC MTNTPVVVREGATVYATGTH AQVEDGRLMEQLLSSVGFCT EVEEDLIDAVTGLSGSGPAY AFTALDALADGGVKMGLPRR LAVRLGAQALLGAAKMLLHS EQHPGQLKDNVSSPGGATI
Function Housekeeping enzyme that catalyzes the last step in proline biosynthesis. Can utilize both NAD and NADP, but has higher affinity for NAD. Involved in the cellular response to oxidative stress.
Protein Name Pyrroline-5-Carboxylate Reductase 1 - Mitochondrial
P5c Reductase 1
P5cr 1
Database Links Reactome: R-HSA-8964539
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Pyrroline-5-Carboxylate Reductase 1 - Mitochondrial antibody
Anti-P5c Reductase 1 antibody
Anti-P5cr 1 antibody
Anti-PYCR1 antibody

Information sourced from Uniprot.org

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