• Western blot analysis of extracts of mouse spleen, using ASC/TMS1 antibody (STJ25245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of A-549 cells using ASC/TMS1 rabbit polyclonal antibody (STJ25245) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse spleen cells using ASC/TMS1 rabbit polyclonal antibody (STJ25245) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat spleen cells using ASC/TMS1 rabbit polyclonal antibody (STJ25245) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

Anti-PYCARD antibody (50-195) (STJ25245)

SKU:
STJ25245

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ASC/TMS1 (50-195) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PYCARD
Gene ID: 29108
Uniprot ID: ASC_HUMAN
Immunogen Region: 50-195
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-195 of human ASC/TMS1 (NP_037390.2).
Immunogen Sequence: LDLTDKLVSFYLETYGAELT ANVLRDMGLQEMAGQLQAAT HQGSGAAPAGIQAPPQSAAK PGLHFIDQHRAALIARVTNV EWLLDALYGKVLTDEQYQAV RAEPTNPSKMRKLFSFTPAW NWTCKDLLLQALRESQSYLV EDLERS
Tissue Specificity Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Expressed in lung epithelial cells (at protein level). Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T-cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells.
Post Translational Modifications Phosphorylated. 'Lys-63'-linked polyubiquitination by TRAF3 is critical for speck formation and inflammasome activation.
Function Functions as key mediator in apoptosis and inflammation. Promotes caspase-mediated apoptosis involving predominantly caspase-8 and also caspase-9 in a probable cell type-specific manner. Involved in activation of the mitochondrial apoptotic pathway, promotes caspase-8-dependent proteolytic maturation of BID independently of FADD in certain cell types and also mediates mitochondrial translocation of BAX and activates BAX-dependent apoptosis coupled to activation of caspase-9, -2 and -3. Involved in innate immune response by acting as an integral adapter in the assembly of various inflammasomes (NLRP1, NLRP2, NLRP3, NLRP6, AIM2 and probably IFI16) which recruit and activate caspase-1 leading to processing and secretion of pro-inflammatory cytokines. Caspase-1-dependent inflammation leads to macrophage pyroptosis, a form of cell death. The function as activating adapter in different types of inflammasomes is mediated by the pyrin and CARD domains and their homotypic interactions. Clustered PYCARD nucleates the formation of caspase-1 filaments through the interaction of their respective CARD domains, acting as a platform for of caspase-1 polymerization. In the NLRP1 and NLRC4 inflammasomes seems not be required but facilitates the processing of procaspase-1. In cooperation with NOD2 involved in an inflammasome activated by bacterial muramyl dipeptide leading to caspase-1 activation. May be involved in RIGI-triggered pro-inflammatory responses and inflammasome activation. In collaboration with AIM2 which detects cytosolic double-stranded DNA may also be involved in a caspase-1-independent cell death that involves caspase-8. In adaptive immunity may be involved in maturation of dendritic cells to stimulate T-cell immunity and in cytoskeletal rearrangements coupled to chemotaxis and antigen uptake may be involved in post-transcriptional regulation of the guanine nucleotide exchange factor DOCK2.the latter function is proposed to involve the nuclear form. Also involved in transcriptional activation of cytokines and chemokines independent of the inflammasome.this function may involve AP-1, NF-kappa-B, MAPK and caspase-8 signaling pathways. For regulation of NF-kappa-B activating and inhibiting functions have been reported. Modulates NF-kappa-B induction at the level of the IKK complex by inhibiting kinase activity of CHUK and IKBK. Proposed to compete with RIPK2 for association with CASP1 thereby down-regulating CASP1-mediated RIPK2-dependent NF-kappa-B activation and activating interleukin-1 beta processing. Modulates host resistance to DNA virus infection, probably by inducing the cleavage of and inactivating CGAS in presence of cytoplasmic double-stranded DNA. Isoform 2: May have a regulating effect on the function as inflammasome adapter. Isoform 3: Seems to inhibit inflammasome-mediated maturation of interleukin-1 beta.
Protein Name Apoptosis-Associated Speck-Like Protein Containing A Card
Hasc
Caspase Recruitment Domain-Containing Protein 5
Pyd And Card Domain-Containing Protein
Target Of Methylation-Induced Silencing 1
Database Links Reactome: R-HSA-5660668
Reactome: R-HSA-6798695
Reactome: R-HSA-844456
Reactome: R-HSA-844615
Reactome: R-HSA-9660826
Reactome: R-HSA-9692916
Cellular Localisation Cytoplasm
Inflammasome
Endoplasmic Reticulum
Mitochondrion
Nucleus
Upstream Of Caspase Activation
A Redistribution From The Cytoplasm To The Aggregates Occurs
These Appear As Hollow
Perinuclear Spherical
Ball-Like Structures
Upon Nlrp3 Inflammasome Activation Redistributes To The Perinuclear Space Localizing To Endoplasmic Reticulum And Mitochondria
Localized Primarily To The Nucleus In Resting Monocytes/Macrophages And Rapidly Redistributed To The Cytoplasm Upon Pathogen Infection
Localized To Large Cytoplasmic Aggregate Appearing As A Speck Containing Aim2
Pycard
Casp8 And Bacterial Dna After Infection With Francisella Tularensis
Golgi Apparatus Membrane
(Microbial Infection) Upon Hrsv Infection
The Protein Is Mainly Located In Lipid Rafts In The Golgi Membrane
Alternative Antibody Names Anti-Apoptosis-Associated Speck-Like Protein Containing A Card antibody
Anti-Hasc antibody
Anti-Caspase Recruitment Domain-Containing Protein 5 antibody
Anti-Pyd And Card Domain-Containing Protein antibody
Anti-Target Of Methylation-Induced Silencing 1 antibody
Anti-PYCARD antibody
Anti-ASC antibody
Anti-CARD5 antibody
Anti-TMS1 antibody

Information sourced from Uniprot.org

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