Anti-PUS1 antibody (308-427) (STJ116327)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PUS1 (308-427) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PUS1 |
| Gene ID | 80324 |
| Uniprot ID | PUS1_HUMAN |
| Immunogen Region | 308-427 |
| Immunogen Sequence | YAPESVLERSWGTEKVDVPK APGLGLVLERVHFEKYNQRF GNDGLHEPLDWAQEEGKVAA FKEEHIYPTIIGTERDERSM AQWLSTLPIHNFSATALTAG GTGAKVPSPLEGSEGDGDTD |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 308-427 of human PUS1 (NP_079491.2). |
Additional Info
| Tissue Specificity | Widely expressed. High levels of expression found in brain and skeletal muscle. |
| Function | Pseudouridylate synthase that catalyzes pseudouridylation of tRNAs and mRNAs. Acts on positions 27/28 in the anticodon stem and also positions 34 and 36 in the anticodon of an intron containing tRNA. Also catalyzes pseudouridylation of mRNAs: mediates pseudouridylation of mRNAs with the consensus sequence 5'-UGUAG-3'. Acts as a regulator of pre-mRNA splicing by mediating pseudouridylation of pre-mRNAs at locations associated with alternatively spliced regions. Pseudouridylation of pre-mRNAs near splice sites directly regulates mRNA splicing and mRNA 3'-end processing. Involved in regulation of nuclear receptor activity through pseudouridylation of SRA1 mRNA. |
| Protein Name | Pseudouridylate Synthase 1 HomologTrna Pseudouridine Synthase 1Trna Pseudouridine(38-40 SynthaseTrna Pseudouridylate Synthase ITrna-Uridine Isomerase I |
| Database Links | Reactome: R-HSA-6782315 Q9Y606-2Reactome: R-HSA-6787450 Q9Y606-1 |
| Cellular Localisation | Isoform 1: MitochondrionIsoform 2: NucleusCytoplasm |
| Alternative Antibody Names | Anti-Pseudouridylate Synthase 1 Homolog antibodyAnti-Trna Pseudouridine Synthase 1 antibodyAnti-Trna Pseudouridine(38-40 Synthase antibodyAnti-Trna Pseudouridylate Synthase I antibodyAnti-Trna-Uridine Isomerase I antibodyAnti-PUS1 antibodyAnti-PP8985 antibody |
Information sourced from Uniprot.org