Anti-PTPRN antibody (STJA0007472)

SKU:
STJA0007472

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Receptor-type tyrosine-protein phosphatase-like N is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPRN
Gene ID: 5798
Uniprot ID: PTPRN_HUMAN
Specificity: This antibody detects endogenous levels of IA-2/PTPRN at Human, Rat
Immunogen: Synthesized peptide derived from human IA-2/PTPRN
Post Translational Modifications N-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. Subject to proteolytic cleavage at multiple sites. Subject to cleavage on a pair of basic residues. On exocytosis of secretory granules in pancreatic beta-cells ICA512-TMF is transiently inserted in the plasma-membrane and cleaved by mu-type calpain CPN1 to yield ICA512-CCF. Sumoylated at two sites including Lys-754. Sumoylation decreases interaction with STAT5.
Function Plays a role in vesicle-mediated secretory processes. Required for normal accumulation of secretory vesicles in hippocampus, pituitary and pancreatic islets. Required for the accumulation of normal levels of insulin-containing vesicles and preventing their degradation. Plays a role in insulin secretion in response to glucose stimuli. Required for normal accumulation of the neurotransmitters norepinephrine, dopamine and serotonin in the brain. In females, but not in males, required for normal accumulation and secretion of pituitary hormones, such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Required to maintain normal levels of renin expression and renin release. Seems to lack intrinsic enzyme activity. May regulate catalytic active protein-tyrosine phosphatases such as PTPRA through dimerization. ICA512-transmembrane fragment: ICA512-TMF regulates dynamics and exocytosis of insulin secretory granules (SGs).binding of ICA512-TMF to SNTB2/beta-2-syntrophin is proposed to restrain SGs mobility and exocytosis by tethering them to the actin cytoskeleton depending on UTRN.the function is inhibited by cytoplasmic ICA512-CFF dimerizing with ICA512-TMF and displacing SNTB2. ICA512-cleaved cytosolic fragment: ICA512-CCF translocated to the nucleus promotes expression of insulin and other granule-related genes.the function implicates binding to and regulating activity of STAT5B probably by preventing its dephosphorylation and potentially by inducing its sumoylation by recruiting PIAS4. Enhances pancreatic beta-cell proliferation by converging with signaling by STAT5B and STAT3. ICA512-CCF located in the cytoplasm regulates dynamics and exocytosis of insulin secretory granules (SGs) by dimerizing with ICA512-TMF and displacing SNTB2 thus enhancing SGs mobility and exocytosis.
Protein Name Receptor-Type Tyrosine-Protein Phosphatase-Like N
R-Ptp-N
Islet Cell Antigen 512
Ica 512
Islet Cell Autoantigen 3
Ptp Ia-2 Cleaved Into - Ica512-N-Terminal Fragment
Ica512-Ntf - Ica512-Transmembrane Fragment
Ica512-Tmf - Ica512-Cleaved Cytosolic Fragment
Ica512-Ccf
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Cytoplasmic Vesicle
Secretory Vesicle Membrane
Perikaryon
Cell Projection
Axon
Synapse
Cell Membrane
Endosome
Detected On Neuronal Secretory Vesicles
But Not On Synaptic Vesicles
Colocalizes With Insulin-Containing Secretory Granules
Primarily Detected On Secretory Vesicle Membranes
Transiently Found At The Cell Membrane
When Secretory Vesicles Fuse With The Cell Membrane To Release Their Cargo
Is Then Endocytosed And Recycled To Secretory Vesicles Via The Golgi Apparatus Membranes
Ica512-Transmembrane Fragment: Cytoplasmic Vesicle
Ica512-Cleaved Cytosolic Fragment: Nucleus
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Phosphatase-Like N antibody
Anti-R-Ptp-N antibody
Anti-Islet Cell Antigen 512 antibody
Anti-Ica 512 antibody
Anti-Islet Cell Autoantigen 3 antibody
Anti-Ptp Ia-2 Cleaved Into - Ica512-N-Terminal Fragment antibody
Anti-Ica512-Ntf - Ica512-Transmembrane Fragment antibody
Anti-Ica512-Tmf - Ica512-Cleaved Cytosolic Fragment antibody
Anti-Ica512-Ccf antibody
Anti-PTPRN antibody
Anti-ICA3 antibody
Anti-ICA512 antibody

Information sourced from Uniprot.org

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