• Jurkat whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD45 (ABT-CD45) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: JurkatPredicted band size: 200kDaObserved band size: 200kDa
  • Human diffuse large B-cell lymphoma tissue was stained with Anti-CD45 (ABT-CD45) Antibody
  • Human diffuse large B-cell lymphoma tissue was stained with Anti-CD45 (ABT-CD45) Antibody
  • Human hepatocellular carcinoma tissue was stained with Anti-CD45 (ABT-CD45) Antibody
  • Human rectal carcinoma tissue was stained with Anti-CD45 (ABT-CD45) Antibody
  • Human spleen tissue was stained with Anti-CD45 (ABT-CD45) Antibody

Anti-PTPRC antibody (500-600 aa) [ABT-CD45] (STJ196813)

SKU:
STJ196813

Current Stock:
Host: Mouse
Applications: IHC/WB/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Receptor-type tyrosine-protein phosphatase C (500-600 aa) is suitable for use in Immunohistochemistry, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Monoclonal
Clone ID: ABT-CD45
Conjugation: Unconjugated
Isotype: IgG2bk
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:200-1000
IF-P FC 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTPRC
Gene ID: 5788
Uniprot ID: PTPRC_HUMAN
Immunogen Region: 500-600 aa
Specificity: This antibody detects endogenous levels of human CD45 (LCA). Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human CD45 (LCA) at the amino acid range 500-600
Post Translational Modifications Heavily N- and O-glycosylated.
Function Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation.
Protein Name Receptor-Type Tyrosine-Protein Phosphatase C
Leukocyte Common Antigen
L-Ca
T200
Cd Antigen Cd45
Database Links Reactome: R-HSA-202427
Reactome: R-HSA-416700
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Colocalized With Dpp4 In Membrane Rafts
Alternative Antibody Names Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibody
Anti-Leukocyte Common Antigen antibody
Anti-L-Ca antibody
Anti-T200 antibody
Anti-Cd Antigen Cd45 antibody
Anti-PTPRC antibody
Anti-CD45 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance