Host: |
Mouse |
Applications: |
IHC/WB/IF/FC |
Reactivity: |
Human |
Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: |
Mouse monoclonal antibody anti-Receptor-type tyrosine-protein phosphatase C (500-600 aa) is suitable for use in Immunohistochemistry, Western Blot, Immunofluorescence and Flow Cytometry research applications. |
Clonality: |
Monoclonal |
Clone ID: |
ABT-CD45 |
Conjugation: |
Unconjugated |
Isotype: |
IgG2bk |
Formulation: |
Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
Purification: |
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
Dilution Range: |
IHC-P 1:100-500WB 1:200-1000IF-P FC 1:50-200 |
Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: |
PTPRC |
Gene ID: |
5788 |
Uniprot ID: |
PTPRC_HUMAN |
Immunogen Region: |
500-600 aa |
Specificity: |
This antibody detects endogenous levels of human CD45 (LCA). Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section |
Immunogen: |
Synthesized peptide derived from the human CD45 (LCA) at the amino acid range 500-600 |
Post Translational Modifications | Heavily N- and O-glycosylated. |
Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. |
Protein Name | Receptor-Type Tyrosine-Protein Phosphatase CLeukocyte Common AntigenL-CaT200Cd Antigen Cd45 |
Database Links | Reactome: R-HSA-202427Reactome: R-HSA-416700Reactome: R-HSA-6798695 |
Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane RaftColocalized With Dpp4 In Membrane Rafts |
Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibodyAnti-Leukocyte Common Antigen antibodyAnti-L-Ca antibodyAnti-T200 antibodyAnti-Cd Antigen Cd45 antibodyAnti-PTPRC antibodyAnti-CD45 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance