Anti-PTPRA antibody (175-295) (STJ114202)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PTPRA (175-295) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:5000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PTPRA |
| Gene ID | 5786 |
| Uniprot ID | PTPRA_HUMAN |
| Immunogen Region | 175-295 |
| Immunogen Sequence | GSHSNSFRLSNGRTEDVEPQ SVPLLARSPSTNRKYPPLPV DKLEEEINRRMADDNKLFRE EFNALPACPIQATCEAASKE ENKEKNRYVNILPYDHSRVH LTPVEGVPDSDYINASFING Y |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 175-295 of human PTPRA (NP_543030.1). |
Additional Info
| Post Translational Modifications | Integrin binding to extracellular matrix induces phosphorylation at Tyr-798 which induces PTPRA localization and recruitment of BCAR3, BCAR1 and CRK to focal adhesions. |
| Function | Tyrosine protein phosphatase which is involved in integrin-mediated focal adhesion formation. Following integrin engagement, specifically recruits BCAR3, BCAR1 and CRK to focal adhesions thereby promoting SRC-mediated phosphorylation of BRAC1 and the subsequent activation of PAK and small GTPase RAC1 and CDC42. |
| Protein Name | Receptor-Type Tyrosine-Protein Phosphatase AlphaProtein-Tyrosine Phosphatase AlphaR-Ptp-Alpha |
| Database Links | Reactome: R-HSA-375165Reactome: R-HSA-5673001 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinCell JunctionFocal AdhesionLocalizes To Focal Adhesion Sites Following Integrin Engagement |
| Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Phosphatase Alpha antibodyAnti-Protein-Tyrosine Phosphatase Alpha antibodyAnti-R-Ptp-Alpha antibodyAnti-PTPRA antibodyAnti-PTPA antibodyAnti-PTPRL2 antibody |
Information sourced from Uniprot.org