• Western blot analysis of lysates from LOVO cells, using Breast Tumor Kinase Antibody. The lane on the right is blocked with the synthesized peptide.
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-PTK6 antibody (10-59 aa) (STJ91891)

SKU:
STJ91891

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Protein-tyrosine kinase 6 (10-59 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTK6
Gene ID: 5753
Uniprot ID: PTK6_HUMAN
Immunogen Region: 10-59 aa
Specificity: Brk Polyclonal Antibody detects endogenous levels of Brk protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Breast Tumor Kinase at the amino acid range 10-59
Post Translational Modifications Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity.
Function Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins: KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF.transcription factors: STAT3 and STAT5A/B and a variety of signaling molecules: ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways. Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.
Protein Name Protein-Tyrosine Kinase 6
Breast Tumor Kinase
Tyrosine-Protein Kinase Brk
Database Links Reactome: R-HSA-187577
Reactome: R-HSA-69231
Reactome: R-HSA-8847993
Reactome: R-HSA-8849468
Reactome: R-HSA-8849469
Reactome: R-HSA-8849470
Reactome: R-HSA-8849471
Reactome: R-HSA-8849472
Reactome: R-HSA-8849473
Reactome: R-HSA-8849474
Reactome: R-HSA-8857538
Reactome: R-HSA-9707564
Cellular Localisation Cytoplasm
Nucleus
Cell Projection
Ruffle
Membrane
Colocalizes With Khdrbs1
Khdrbs2 Or Khdrbs3
Within The Nucleus
Nuclear Localization In Epithelial Cells Of Normal Prostate But Cytoplasmic Localization In Cancer Prostate
Alternative Antibody Names Anti-Protein-Tyrosine Kinase 6 antibody
Anti-Breast Tumor Kinase antibody
Anti-Tyrosine-Protein Kinase Brk antibody
Anti-PTK6 antibody
Anti-BRK antibody

Information sourced from Uniprot.org

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