Anti-PTGS1 antibody (1-180) (STJ29480)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PTGS1 (1-180) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:100-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PTGS1 |
| Gene ID | 5742 |
| Uniprot ID | PGH1_HUMAN |
| Immunogen Region | 1-180 |
| Immunogen Sequence | MLMRLVLTVRSNLIPSPPTY NSAHDYISWESFSNVSYYTR ILPSVPKDCPTPMGTKGKKQ LPDAQLLARRFLLRRKFIPD PQGTNLMFAFFAQHFTHQFF KTSGKMGPGFTKALGHGVDL GHIYGDNLERQYQLRLFKDG KLKYQVLDGEMYPPSVEEAP VLMHYPRGIPPQSQMAVGQE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human COX1/COX1/PTGS1 (NP_001258094.1). |
Additional Info
| Function | Dual cyclooxygenase and peroxidase that plays an important role in the biosynthesis pathway of prostanoids, a class of C20 oxylipins mainly derived from arachidonate ((5Z,8Z,11Z,14Z)-eicosatetraenoate, AA, C20:4(n-6)), with a particular role in the inflammatory response. The cyclooxygenase activity oxygenates AA to the hydroperoxy endoperoxide prostaglandin G2 (PGG2), and the peroxidase activity reduces PGG2 to the hydroxy endoperoxide prostaglandin H2 (PGH2), the precursor of all 2-series prostaglandins and thromboxanes. This complex transformation is initiated by abstraction of hydrogen at carbon 13 (with S-stereochemistry), followed by insertion of molecular O2 to form the endoperoxide bridge between carbon 9 and 11 that defines prostaglandins. The insertion of a second molecule of O2 (bis-oxygenase activity) yields a hydroperoxy group in PGG2 that is then reduced to PGH2 by two electrons. Involved in the constitutive production of prostanoids in particular in the stomach and platelets. In gastric epithelial cells, it is a key step in the generation of prostaglandins, such as prostaglandin E2 (PGE2), which plays an important role in cytoprotection. In platelets, it is involved in the generation of thromboxane A2 (TXA2), which promotes platelet activation and aggregation, vasoconstriction and proliferation of vascular smooth muscle cells (Probable). Can also use linoleate (LA, (9Z,12Z)-octadecadienoate, C18:2(n-6)) as substrate and produce hydroxyoctadecadienoates (HODEs) in a regio- and stereospecific manner, being (9R)-HODE ((9R)-hydroxy-(10E,12Z)-octadecadienoate) and (13S)-HODE ((13S)-hydroxy-(9Z,11E)-octadecadienoate) its major products. |
| Protein Name | Prostaglandin G/H Synthase 1Cyclooxygenase-1Cox-1Prostaglandin H2 Synthase 1Pgh Synthase 1Pghs-1Phs 1Prostaglandin-Endoperoxide Synthase 1 |
| Database Links | Reactome: R-HSA-140180Reactome: R-HSA-2162123 |
| Cellular Localisation | Microsome MembranePeripheral Membrane ProteinEndoplasmic Reticulum Membrane |
| Alternative Antibody Names | Anti-Prostaglandin G/H Synthase 1 antibodyAnti-Cyclooxygenase-1 antibodyAnti-Cox-1 antibodyAnti-Prostaglandin H2 Synthase 1 antibodyAnti-Pgh Synthase 1 antibodyAnti-Pghs-1 antibodyAnti-Phs 1 antibodyAnti-Prostaglandin-Endoperoxide Synthase 1 antibodyAnti-PTGS1 antibodyAnti-COX1 antibody |
Information sourced from Uniprot.org