• Western blot analysis of extracts of various cell lines, using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) rabbit monoclonal antibody (STJ11102414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of U-2 OS cells using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) Rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human placenta using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of NIH-3T3 cells using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) Rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) Rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded human placenta using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) Rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) rabbit monoclonal antibody (STJ11102414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) Rabbit monoclonal antibody (STJ11102414) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PTGER2 antibody (259-358) [S4MR] (STJ11102414)

SKU:
STJ11102414

€183.13 - €799.38
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PTGER2 (259-358) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTGER2
Gene ID: 5732
Uniprot ID: PE2R2_HUMAN
Immunogen Region: 259-358
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 259-358 of human PGE receptor EP2 (PTGER2) (P43116).
Immunogen Sequence: ETDHLILLAIMTITFAVCSL PFTIFAYMNETSSRKEKWDL QALRFLSINSIIDPWVFAIL RPPVLRLMRSVLCCRISLRT QDATQTSCSTQSDASKQADL
Tissue Specificity Placenta and lung.
Function Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Protein Name Prostaglandin E2 Receptor Ep2 Subtype
Pge Receptor Ep2 Subtype
Pge2 Receptor Ep2 Subtype
Prostanoid Ep2 Receptor
Database Links Reactome: R-HSA-391908
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Prostaglandin E2 Receptor Ep2 Subtype antibody
Anti-Pge Receptor Ep2 Subtype antibody
Anti-Pge2 Receptor Ep2 Subtype antibody
Anti-Prostanoid Ep2 Receptor antibody
Anti-PTGER2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance