Western blot analysis of various lysates using PGE Receptor EP2 (PGE Receptor EP2 (PGE receptor EP2 (PTGER2) ) ) Rabbit mAb (STJ11102414) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-PTGER2 antibody (259-358) [S4MR] (STJ11102414)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11102414
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General Information

Short DescriptionRabbit monoclonal PTGER2 (259-358) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS4MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:1000-1:6000
IHC-P:1:100-1:1000
IF/ICC:1:100-1:400
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPTGER2
Gene ID5732
Uniprot IDPE2R2_HUMAN
Immunogen Region259-358
Immunogen SequenceETDHLILLAIMTITFAVCSL PFTIFAYMNETSSRKEKWDL QALRFLSINSIIDPWVFAIL RPPVLRLMRSVLCCRISLRT QDATQTSCSTQSDASKQADL
SpecificityA synthetic peptide corresponding to a sequence within amino acids 259-358 of human PGE receptor EP2 (PTGER2) (P43116).

Additional Info

Tissue Specificity Placenta and lung.
Function Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle.
Protein Name Prostaglandin E2 Receptor Ep2 Subtype
Pge Receptor Ep2 Subtype
Pge2 Receptor Ep2 Subtype
Prostanoid Ep2 Receptor
Database Links Reactome: R-HSA-391908
Reactome: R-HSA-418555
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Prostaglandin E2 Receptor Ep2 Subtype antibody
Anti-Pge Receptor Ep2 Subtype antibody
Anti-Pge2 Receptor Ep2 Subtype antibody
Anti-Prostanoid Ep2 Receptor antibody
Anti-PTGER2 antibody

Information sourced from Uniprot.org

Citations

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