||Human, Mouse, Rat
||Rabbit polyclonal anti-PTEN antibody is suitable for use in Western Blot and Immunofluorescence.
||FOR RESEARCH USE ONLY (RUO).
||PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
||Store at-20°C. Avoid freeze/thaw cycles.
||WB: 1:500-1:2000IF: 1:50-1:200
||Recombinant fusion protein containing a sequence corresponding to amino acids 209-403 of human PTEN (NP_000305.3).
| Tissue Specificity || Expressed at a relatively high level in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney and pancreas. |
| Post Translational Modifications || Constitutively phosphorylated by CK2 under normal conditions. Phosphorylated in vitro by MAST1, MAST2, MAST3 and STK11. Phosphorylation results in an inhibited activity towards PIP3. Phosphorylation can both inhibit or promote PDZ-binding. Phosphorylation at Tyr-336 by FRK/PTK5 protects this protein from ubiquitin-mediated degradation probably by inhibiting its binding to NEDD4. Phosphorylation by ROCK1 is essential for its stability and activity. Phosphorylation by PLK3 promotes its stability and prevents its degradation by the proteasome. Monoubiquitinated.monoubiquitination is increased in presence of retinoic acid. Deubiquitinated by USP7.leading to its nuclear exclusion. Monoubiquitination of one of either Lys-13 and Lys-289 amino acid is sufficient to modulate PTEN compartmentalization. Ubiquitinated by XIAP/BIRC4. |
| Function || Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3,4,5-trisphosphate, phosphatidylinositol 3,4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1,3,4,5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3,4,5)P3 > PtdIns(3,4)P2 > PtdIns3P > Ins(1,3,4,5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. Plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue. The nuclear monoubiquitinated form possesses greater apoptotic potential, whereas the cytoplasmic nonubiquitinated form induces less tumor suppressive ability. In motile cells, suppresses the formation of lateral pseudopods and thereby promotes cell polarization and directed movement. Isoform alpha: Functional kinase, like isoform 1 it antagonizes the PI3K-AKT/PKB signaling pathway. Plays a role in mitochondrial energetic metabolism by promoting COX activity and ATP production, via collaboration with isoform 1 in increasing protein levels of PINK1. |
| Protein Name || Phosphatidylinositol 3 -4 -5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase Pten Mutated In Multiple Advanced Cancers 1Phosphatase And Tensin Homolog |
| Database Links || Reactome: R-HSA-1660499Reactome: R-HSA-1855204Reactome: R-HSA-199418Reactome: R-HSA-202424Reactome: R-HSA-5628897Reactome: R-HSA-5674404Reactome: R-HSA-5689880Reactome: R-HSA-5689896Reactome: R-HSA-8943723Reactome: R-HSA-8948747Reactome: R-HSA-8948751Reactome: R-HSA-8986944 |
| Cellular Localisation || CytoplasmNucleusPml BodyMonoubiquitinated Form Is NuclearNonubiquitinated Form Is CytoplasmicColocalized With Pml And Usp7 In Pml Nuclear BodiesXiap/Birc4 Promotes Its Nuclear LocalizationIsoform Alpha: SecretedMay Be Secreted Via A Classical Signal Peptide And Reenter Into Cells With The Help Of A Poly-Arg Motif |
| Alternative Antibody Names || Anti-Phosphatidylinositol 3 -4 -5-Trisphosphate 3-Phosphatase And Dual-Specificity Protein Phosphatase Pten antibodyAnti-Mutated In Multiple Advanced Cancers 1 antibodyAnti-Phosphatase And Tensin Homolog antibodyAnti-PTEN antibodyAnti-MMAC1 antibodyAnti-TEP1 antibody |
Information sourced from Uniprot.org
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