• Western blot analysis of extracts of various cell lines, using PTCH1 antibody (STJ111033) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded Rat ovary using PTCH1 antibody (STJ111033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human tonsil using PTCH1 antibody (STJ111033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human breast using PTCH1 antibody (STJ111033) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using PTCH1 antibody (STJ111033) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (STJ111033) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse embryos cells using PTCH1 antibody (STJ111033) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-PTCH1 antibody (1214-1312) (STJ111033)

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STJ111033

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PTCH1 (1214-1312) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PTCH1
Gene ID: 5727
Uniprot ID: PTC1_HUMAN
Immunogen Region: 1214-1312
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1214-1312 of human PTCH1 (NP_000255.2).
Immunogen Sequence: THSGSDSSDSEYSSQTTVSG LSEELRHYEAQQGAGGPAHQ VIVEATENPVFAHSTVVHPE SRHHPPSNPRQQPHLDSGSL PPGRQGQQPRRDPPREGLW
Tissue Specificity In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.
Post Translational Modifications Glycosylation is necessary for SHH binding. In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.
Function Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
Protein Name Protein Patched Homolog 1
Ptc
Ptc1
Database Links Reactome: R-HSA-373080
Reactome: R-HSA-5610787
Reactome: R-HSA-5632681
Reactome: R-HSA-5632684
Reactome: R-HSA-5635838
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Protein Patched Homolog 1 antibody
Anti-Ptc antibody
Anti-Ptc1 antibody
Anti-PTCH1 antibody
Anti-PTCH antibody

Information sourced from Uniprot.org

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