• Western blot analysis of extracts of various cell lines, using PSPH antibody (STJ110233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PSPH antibody (STJ110233) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry of paraffin-embedded human stomach using PSPH antibody (STJ110233) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat brain using PSPH antibody (STJ110233) at dilution of 1:100 (40x lens).

Anti-PSPH antibody (1-225) (STJ110233)

SKU:
STJ110233

€140.63 - €653.13
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSPH (1-225) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSPH
Gene ID: 5723
Uniprot ID: SERB_HUMAN
Immunogen Region: 1-225
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human PSPH (NP_004568.2).
Immunogen Sequence: MVSHSELRKLFYSADAVCFD VDSTVIREEGIDELAKICGV EDAVSEMTRRAMGGAVPFKA ALTERLALIQPSREQVQRLI AEQPPHLTPGIRELVSRLQE RNVQVFLISGGFRSIVEHVA SKLNIPATNVFANRLKFYFN GEYAGFDETQPTAESGGKGK VIKLLKEKFHFKKIIMIGDG ATDMEACPPADAFIGFGGNV IRQQVKDNAKWYITDFVELL GELEE
Function Catalyzes the last irreversible step in the biosynthesis of L-serine from carbohydrates, the dephosphorylation of O-phospho-L-serine to L-serine. L-serine can then be used in protein synthesis, to produce other amino acids, in nucleotide metabolism or in glutathione synthesis, or can be racemized to D-serine, a neuromodulator. May also act on O-phospho-D-serine (Probable).
Protein Name Phosphoserine Phosphatase
Psp
Pspase
L-3-Phosphoserine Phosphatase
O-Phosphoserine Phosphohydrolase
Database Links Reactome: R-HSA-977347
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Phosphoserine Phosphatase antibody
Anti-Psp antibody
Anti-Pspase antibody
Anti-L-3-Phosphoserine Phosphatase antibody
Anti-O-Phosphoserine Phosphohydrolase antibody
Anti-PSPH antibody

Information sourced from Uniprot.org

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