• Western blot analysis of extracts of various cell lines, using PSPC1 antibody (STJ116347) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 200 Mu g extracts of A-549 cells using 3 Mu g PSPC1 antibody (STJ116347). Western blot was performed from the immunoprecipitate using PSPC1 antibody (STJ116347) at a dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded mouse testis using PSPC1 rabbit polyclonal antibody (STJ116347) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PSPC1 in paraffin-embedded Human esophageal using PSPC1 Rabbit polyclonal antibody (STJ116347) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Rat brain using PSPC1 rabbit polyclonal antibody (STJ116347) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PSPC1 in paraffin-embedded Rat brain using PSPC1 Rabbit polyclonal antibody (STJ116347) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded Human esophageal using PSPC1 rabbit polyclonal antibody (STJ116347) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of PSPC1 in paraffin-embedded Mouse testis using PSPC1 Rabbit polyclonal antibody (STJ116347) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug PSPC1 antibody (STJ116347). Western blot was performed from the immunoprecipitate using PSPC1 antibody (STJ116347) at a dilition of 1:1000.
  • Western blot analysis of various lysates using PSPC1 Rabbit polyclonal antibody (STJ116347) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PSPC1 antibody (1-70) (STJ116347)

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STJ116347

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PSPC1 (1-70) is suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSPC1
Gene ID: 55269
Uniprot ID: PSPC1_HUMAN
Immunogen Region: 1-70
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human PSPC1 (NP_001035879.1).
Immunogen Sequence: MMLRGNLKQVRIEKNPARLR ALESAVGESEPAAAAAMALA LAGEPAPPAPAPPEDHPDEE MGFTIDIKSF
Tissue Specificity Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart.
Function Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line. Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Together with NONO, required for the formation of nuclear paraspeckles. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Protein Name Paraspeckle Component 1
Paraspeckle Protein 1
Cellular Localisation Nucleus
Nucleolus
Nucleus Matrix
Cytoplasm
Nucleus Speckle
In Punctate Subnuclear Structures Often Located Adjacent To Splicing Speckles
Called Paraspeckles
Colocalizes With Nono And Sfpq In Paraspeckles And Perinucleolar Caps In An Rna-Dependent Manner
May Cycle Between Paraspeckles And Nucleolus
In Telophase
When Daughter Nuclei Form
Localizes To Perinucleolar Caps
Alternative Antibody Names Anti-Paraspeckle Component 1 antibody
Anti-Paraspeckle Protein 1 antibody
Anti-PSPC1 antibody
Anti-PSP1 antibody

Information sourced from Uniprot.org

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