Anti-PSME3 antibody (200-254) (STJ114570)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PSME3 (200-254) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PSME3 |
| Gene ID | 10197 |
| Uniprot ID | PSME3_HUMAN |
| Immunogen Region | 200-254 |
| Immunogen Sequence | EDYRRTVTEIDEKEYISLRL IISELRNQYVTLHDMILKNI EKIKRPRSSNAETLY |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-254 of human PSME3 (NP_005780.2). |
Additional Info
| Post Translational Modifications | Phosphorylated by MAP3K3. Phosphorylation at Ser-247 promotes its association with CCAR2. Acetylation at the major site Lys-195 is important for oligomerization and ability to degrade its target substrates. Deacetylated by SIRT1. |
| Function | Subunit of the 11S REG-gamma (also called PA28-gamma) proteasome regulator, a doughnut-shaped homoheptamer which associates with the proteasome. 11S REG-gamma activates the trypsin-like catalytic subunit of the proteasome but inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. Facilitates the MDM2-p53/TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, limiting its accumulation and resulting in inhibited apoptosis after DNA damage. May also be involved in cell cycle regulation. Mediates CCAR2 and CHEK2-dependent SIRT1 inhibition. |
| Protein Name | Proteasome Activator Complex Subunit 311s Regulator Complex Subunit GammaReg-GammaActivator Of Multicatalytic Protease Subunit 3Ki Nuclear AutoantigenProteasome Activator 28 Subunit GammaPa28gPa28gamma |
| Database Links | Reactome: R-HSA-9907900 |
| Cellular Localisation | NucleusCytoplasmLocalizes To The Cytoplasm During Mitosis Following Nuclear Envelope Breakdown At This Distinct Stage Of The Cell Cycle Which Allows Its Interaction With Map3k3 Kinase |
| Alternative Antibody Names | Anti-Proteasome Activator Complex Subunit 3 antibodyAnti-11s Regulator Complex Subunit Gamma antibodyAnti-Reg-Gamma antibodyAnti-Activator Of Multicatalytic Protease Subunit 3 antibodyAnti-Ki Nuclear Autoantigen antibodyAnti-Proteasome Activator 28 Subunit Gamma antibodyAnti-Pa28g antibodyAnti-Pa28gamma antibodyAnti-PSME3 antibody |
Information sourced from Uniprot.org