| Host: |
Mouse |
| Applications: |
WB/IHC/IF/ELISA |
| Reactivity: |
Human/Rat/Monkey |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Mouse monoclonal antibody anti-PC4 and SFRS1-interacting protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
6E4 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG1 |
| Formulation: |
Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PSIP1 |
| Gene ID: |
11168 |
| Uniprot ID: |
PSIP1_HUMAN |
| Specificity: |
LEDGF Monoclonal Antibody detects endogenous levels of LEDGF protein. |
| Immunogen: |
Purified recombinant fragment of human LEDGF expressed in E. Coli. |
| Post Translational Modifications | Citrullinated by PADI4. |
| Function | Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. |
| Protein Name | Pc4 And Sfrs1-Interacting ProteinCll-Associated Antigen Kw-7Dense Fine Speckles 70 Kda ProteinDfs 70Lens Epithelium-Derived Growth FactorTranscriptional Coactivator P75/P52 |
| Database Links | Reactome: R-HSA-162592Reactome: R-HSA-164843Reactome: R-HSA-175567Reactome: R-HSA-177539Reactome: R-HSA-180689Reactome: R-HSA-180910Reactome: R-HSA-9772755 |
| Cellular Localisation | NucleusRemains Chromatin-Associated Throughout The Cell Cycle |
| Alternative Antibody Names | Anti-Pc4 And Sfrs1-Interacting Protein antibodyAnti-Cll-Associated Antigen Kw-7 antibodyAnti-Dense Fine Speckles 70 Kda Protein antibodyAnti-Dfs 70 antibodyAnti-Lens Epithelium-Derived Growth Factor antibodyAnti-Transcriptional Coactivator P75/P52 antibodyAnti-PSIP1 antibodyAnti-DFS70 antibodyAnti-LEDGF antibodyAnti-PSIP2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PSIP1 knockout (KO) 293T cells, using [KO Validated] PSIP1 Rabbit polyclonal antibody (STJ11100010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89083/357543/STJ11100010_1__78803.1713121661.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSIP1 knockout (KO) 293T cells, using [KO Validated] PSIP1 Rabbit polyclonal antibody (STJ11100010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western blot analysis of lysates from wild type (WT) and PSIP1 knockout (KO) 293T cells, using [KO Validated] PSIP1 Rabbit polyclonal antibody (STJ11100074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89144/357594/STJ11100074_1__61366.1713121732.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PSIP1 knockout (KO) 293T cells, using [KO Validated] PSIP1 Rabbit polyclonal antibody (STJ11100074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
