• Western blot analysis of extracts of various cell lines, using PEN2/PSENEN rabbit monoclonal antibody (STJ11102352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Confocal imaging of paraffin-embedded Mouse brain using PEN2/PSENEN Rabbit monoclonal antibody (STJ11102352, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of NIH-3T3 cells using PEN2/PSENEN rabbit monoclonal antibody (STJ11102352) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Confocal imaging of NIH/3T3 cells using PEN2/PSENEN Rabbit monoclonal antibody (STJ11102352, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using PEN2/PSENEN Rabbit monoclonal antibody (STJ11102352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PSENEN antibody (1-101) [S2MR] (STJ11102352)

SKU:
STJ11102352

$197.78 - $863.33
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PEN2 (1-101) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S2MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PSENEN
Gene ID: 55851
Uniprot ID: PEN2_HUMAN
Immunogen Region: 1-101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human PEN2/PSENEN (NP_758844.1)
Immunogen Sequence: MNLERVSNEEKLNLCRKYYL GGFAFLPFLWLVNIFWFFRE AFLVPAYTEQSQIKGYVWRS AVGFLFWVIVLTSWITIFQI YRPRWGALGDYLSFTIPLGT P
Tissue Specificity Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
Function Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (Probable). PSENEN modulates both endoproteolysis of presenilin and gamma-secretase activity.
Protein Name Gamma-Secretase Subunit Pen-2
Presenilin Enhancer Protein 2
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-193692
Reactome: R-HSA-205043
Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2894862
Reactome: R-HSA-2979096
Reactome: R-HSA-3928665
Reactome: R-HSA-9013507
Reactome: R-HSA-9013700
Reactome: R-HSA-9017802
Reactome: R-HSA-977225
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Golgi Apparatus
Golgi Stack Membrane
Cell Membrane
Membrane
Predominantly Located In The Endoplasmic Reticulum And In The Cis-Golgi
Alternative Antibody Names Anti-Gamma-Secretase Subunit Pen-2 antibody
Anti-Presenilin Enhancer Protein 2 antibody
Anti-PSENEN antibody
Anti-PEN2 antibody
Anti-MDS033 antibody

Information sourced from Uniprot.org

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