• Immunofluorescence analysis of C6 cells using PRR5 Rabbit polyclonal antibody (STJ117364) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PRR5 Rabbit polyclonal antibody (STJ117364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates using PRR5 Rabbit polyclonal antibody (STJ117364) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRR5 antibody (190-388) (STJ117364)

SKU:
STJ117364

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRR5 (190-388) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRR5
Gene ID: 55615
Uniprot ID: PRR5_HUMAN
Immunogen Region: 190-388
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-388 of human PRR5 (NP_851850.1).
Immunogen Sequence: SRGVTEDYLRLETLVQKVVS PYLGTYGLHSSEGPFTHSCI LEKRLLRRSRSGDVLAKNPV VRSKSYNTPLLNPVQEHEAE GAAAGGTSIRRHSVSEMTSC PEPQGFSDPPGQGPTGTFRS SPAPHSGPCPSRLYPTTQPP EQGLDPTRSSLPRSSPENLV DQILESVDSDSEGIFIDFGR GRGSGMSDLEGSGGRQSVV
Tissue Specificity Most abundant in kidney and liver. Also highly expressed in brain, spleen, testis and placenta. Overexpressed in several colorectal tumors.
Function Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. May act as a tumor suppressor in breast cancer.
Protein Name Proline-Rich Protein 5
Protein Observed With Rictor-1
Protor-1
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-389357
Reactome: R-HSA-5218920
Reactome: R-HSA-5674400
Reactome: R-HSA-6804757
Alternative Antibody Names Anti-Proline-Rich Protein 5 antibody
Anti-Protein Observed With Rictor-1 antibody
Anti-Protor-1 antibody
Anti-PRR5 antibody
Anti-PROTOR1 antibody
Anti-PP610 antibody

Information sourced from Uniprot.org

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