Anti-PRPF4B antibody (STJ191370)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
General Information
| Short Description | Rabbit polyclonal anti-Serine/threonine-protein kinase PRP4 homolog for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRP4K |
| Gene ID | 8899 |
| Uniprot ID | PRP4K_HUMAN |
| Immunogen | Synthesized peptide derived from part of the human protein |
| Specificity | PRP4B Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Phosphorylated by CLK1. Autophosphorylated.phosphorylation inhibits interaction with its targets, such as PRPF6 or SMARCA4. |
| Function | Serine/threonine kinase involved in spliceosomal assembly as well as mitosis and signaling regulation. Connects chromatin mediated regulation of transcription and pre-mRNA splicing. During spliceosomal assembly, interacts with and phosphorylates PRPF6 and PRPF31, components of the U4/U6-U5 tri-small nuclear ribonucleoprotein (snRNP), to facilitate the formation of the spliceosome B complex. Plays a role in regulating transcription and the spindle assembly checkpoint (SAC). Associates with U5 snRNP and NCOR1 deacetylase complexes which may allow a coordination of pre-mRNA splicing with chromatin remodeling events involved in transcriptional regulation. Associates and probably phosphorylates SMARCA4 and NCOR1. Phosphorylates SRSF1. Associates with kinetochores during mitosis and is necessary for recruitment and maintenance of the checkpoint proteins such as MAD1L1 and MAD12L1 at the kinetochores. Phosphorylates and regulates the activity of the transcription factors such as ELK1 and KLF13. Phosphorylates nuclear YAP1 and WWTR1/TAZ which induces nuclear exclusion and regulates Hippo signaling pathway, involved in tissue growth control. |
| Protein Name | Serine/Threonine-Protein Kinase Prp4 HomologPrp4 KinasePrp4 Pre-Mrna-Processing Factor 4 Homolog |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusChromosomeCentromereKinetochoreLocated Throughout The NucleusExcluding The Nucleolus But Enriched In Multiple Speckles |
| Alternative Antibody Names | Anti-Serine/Threonine-Protein Kinase Prp4 Homolog antibodyAnti-Prp4 Kinase antibodyAnti-Prp4 Pre-Mrna-Processing Factor 4 Homolog antibodyAnti-PRP4K antibodyAnti-KIAA0536 antibodyAnti-PRP4 antibodyAnti-PRP4B antibodyAnti-PRP4H antibodyAnti-PRPF4K antibody |
Information sourced from Uniprot.org