Anti-PRPF3 antibody (1-280) (STJ27435)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRPF3 (1-280) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRPF3 |
| Gene ID | 9129 |
| Uniprot ID | PRPF3_HUMAN |
| Immunogen Region | 1-280 |
| Immunogen Sequence | MALSKRELDELKPWIEKTVK RVLGFSEPTVVTAALNCVGK GMDKKKAADHLKPFLDDSTL RFVDKLFEAVEEGRSSRHSK SSSDRSRKRELKEVFGDDSE ISKESSGVKKRRIPRFEEVE EEPEVIPGPPSESPGMLTKL QIKQMMEAATRQIEERKKQL SFISPPTPQPKTPSSSQPER LPIGNTIQPSQAATFMNDAI EKARKAAELQARIQAQLALK PGLIGNANMVGLANLHAMG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PRPF3 (NP_004689.1). |
Additional Info
| Tissue Specificity | Highly expressed in retina, liver, kidney and blood. Detected at lower levels in heart and brain. |
| Post Translational Modifications | Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex. |
| Function | Plays a role in pre-mRNA splicing as component of the U4/U6-U5 tri-snRNP complex that is involved in spliceosome assembly, and as component of the precatalytic spliceosome (spliceosome B complex). |
| Protein Name | U4/U6 Small Nuclear Ribonucleoprotein Prp3Pre-Mrna-Splicing Factor 3Hprp3U4/U6 Snrnp 90 Kda Protein |
| Database Links | Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleus Speckle |
| Alternative Antibody Names | Anti-U4/U6 Small Nuclear Ribonucleoprotein Prp3 antibodyAnti-Pre-Mrna-Splicing Factor 3 antibodyAnti-Hprp3 antibodyAnti-U4/U6 Snrnp 90 Kda Protein antibodyAnti-PRPF3 antibodyAnti-HPRP3 antibodyAnti-PRP3 antibody |
Information sourced from Uniprot.org