Anti-PRP19 antibody (200-300) {FITC} (STJ502671)

SKU:
STJ502671-100

£546.50
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Host: Rabbit
Applications: ELISA/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRP19 (200-300) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: FITC
Isotype: IgG
Purification: Affinity Purified
Concentration: 0.50-0.75 µg/µl
Dilution Range: WB: 1:500-1:1, 000
DB: 1:2, 000
ELISA: 1:2, 000
Storage Instruction: Store at-20°C for long term storage. Avoid freeze-thaw cycles.
Gene Symbol: Prpf19
Gene ID: 246216
Uniprot ID: PRP19_RAT
Immunogen Region: 200-300
Immunogen: Synthetic peptide taken within amino acid region 200-300 on rat PRP19F protein.
Function Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.
Protein Name Pre-Mrna-Processing Factor 19
Neuronal Differentiation-Related Gene Protein
Prp19/Pso4 Homolog
Ring-Type E3 Ubiquitin Transferase Prp19
Database Links Reactome: R-RNO-6781823
Reactome: -RNO-6782135
Reactome: -RNO-6782210
Reactome: -RNO-72163
Cellular Localisation Nucleus
Nucleoplasm
Cytoplasm
Cytoskeleton
Spindle
Lipid Droplet
Nucleoplasmic In Interphase Cells
Irregularly Distributed In Anaphase Cells
In Prophase Cells
Uniformly Distributed
But Not Associated With Condensing Chromosomes
Found In Extrachromosomal Regions In Metaphase Cells
Mainly Localized To The Mitotic Spindle Apparatus When Chromosomes Segregate During Anaphase
When Nuclei Reform During Late Telophase
Uniformly Distributed In Daughter Cells And Displays No Preferred Association With Decondensing Chromatin
Recruited On Damaged Dna At Sites Of Double-Strand Break
Alternative Antibody Names Anti-Pre-Mrna-Processing Factor 19 antibody
Anti-Neuronal Differentiation-Related Gene Protein antibody
Anti-Prp19/Pso4 Homolog antibody
Anti-Ring-Type E3 Ubiquitin Transferase Prp19 antibody
Anti-Prpf19 antibody
Anti-Prp19 antibody

Information sourced from Uniprot.org

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