• Confocal imaging of paraffin-embedded Mouse liver tissue using Coagulation Protein C Rabbit monoclonal antibody (STJ11102096, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of various lysates using Coagulation Coagulation Protein C Rabbit monoclonal antibody (STJ11102096) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PROC antibody (300-461) [S6MR] (STJ11102096)

SKU:
STJ11102096

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Protein C (300-461) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PROC
Gene ID: 5624
Uniprot ID: PROC_HUMAN
Immunogen Region: 300-461
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 300-461 of human Coagulation Coagulation Protein C (P04070).
Immunogen Sequence: IALLHLAQPATLSQTIVPIC LPDSGLAERELNQAGQETLV TGWGYHSSREKEAKRNRTFV LNFIKIPVVPHNECSEVMSN MVSENMLCAGILGDRQDACE GDSGGPMVASFHGTWFLVGL VSWGEGCGLLHNYGVYTKVS RYLDWIHGHIRDKEAPQKSW AP
Tissue Specificity Plasma.synthesized in the liver.
Post Translational Modifications The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium. N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans. The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
Function Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. Exerts a protective effect on the endothelial cell barrier function.
Protein Name Vitamin K-Dependent Protein C
Anticoagulant Protein C
Autoprothrombin Iia
Blood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide
Database Links Reactome: R-HSA-140837
Reactome: R-HSA-140875
Reactome: R-HSA-159740
Reactome: R-HSA-159763
Reactome: R-HSA-159782
Reactome: R-HSA-202733
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Secreted
Golgi Apparatus
Endoplasmic Reticulum
Alternative Antibody Names Anti-Vitamin K-Dependent Protein C antibody
Anti-Anticoagulant Protein C antibody
Anti-Autoprothrombin Iia antibody
Anti-Blood Coagulation Factor Xiv Cleaved Into - Vitamin K-Dependent Protein C Light Chain - Vitamin K-Dependent Protein C Heavy Chain - Activation Peptide antibody
Anti-PROC antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance