Anti-PROM1 antibody [6H10-F1-C11] (STJ99199)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG2a
ImmunogenPurified recombinant human Prominin-1 protein fragments expressed in E.coli.
General Information
| Short Description | Mouse monoclonal anti-Prominin-1 for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 6H10-F1-C11 |
| Isotype | IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:1000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PROM1 |
| Gene ID | 8842 |
| Uniprot ID | PROM1_HUMAN |
| Immunogen | Purified recombinant human Prominin-1 protein fragments expressed in E.coli. |
| Specificity | This antibody detects endogenous levels of Prominin-1 and does not cross-react with related proteins. |
Additional Info
| Post Translational Modifications | Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. |
| Function | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| Protein Name | Prominin-1Antigen Ac133Prominin-Like Protein 1Cd Antigen Cd133 |
| Database Links | |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell ProjectionMicrovillus MembraneCiliumPhotoreceptor Outer SegmentEndoplasmic ReticulumEndoplasmic Reticulum-Golgi Intermediate CompartmentFound In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal FluidSalivaSeminal Fluid And Urine |
| Alternative Antibody Names | Anti-Prominin-1 antibodyAnti-Antigen Ac133 antibodyAnti-Prominin-Like Protein 1 antibodyAnti-Cd Antigen Cd133 antibodyAnti-PROM1 antibodyAnti-PROML1 antibodyAnti-MSTP061 antibody |
Information sourced from Uniprot.org