• Western blot analysis of extracts of various cell lines, using PRODH antibody (STJ28399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRODH antibody (321-600) (STJ28399)

SKU:
STJ28399

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRODH (321-600) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRODH
Gene ID: 5625
Uniprot ID: PROD_HUMAN
Immunogen Region: 321-600
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 321-600 of human PRODH (NP_057419.4).
Immunogen Sequence: IDSRTKLSKHLVVPNAQTGQ LEPLLSRFTEEEELQMTRML QRMDVLAKKATEMGVRLMVD AEQTYFQPAISRLTLEMQRK FNVEKPLIFNTYQCYLKDAY DNVTLDVELARREGWCFGAK LVRGAYLAQERARAAEIGYE DPINPTYEATNAMYHRCLDY VLEELKHNAKAKVMVASHNE DTVRFALRRMEELGLHPADH QVYFGQLLGMCDQISFPLGQ AGYPVYKYVPYGPVMEVLP
Tissue Specificity Expressed in lung, skeletal muscle and brain, to a lesser extent in heart and kidney, and weakly in liver, placenta and pancreas.
Function Converts proline to delta-1-pyrroline-5-carboxylate.
Protein Name Proline Dehydrogenase 1 - Mitochondrial
Proline Oxidase
Proline Oxidase 2
P53-Induced Gene 6 Protein
Database Links Reactome: R-HSA-70688
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Proline Dehydrogenase 1 - Mitochondrial antibody
Anti-Proline Oxidase antibody
Anti-Proline Oxidase 2 antibody
Anti-P53-Induced Gene 6 Protein antibody
Anti-PRODH antibody
Anti-PIG6 antibody
Anti-POX2 antibody
Anti-PRODH2 antibody

Information sourced from Uniprot.org

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