Anti-PRNP antibody [3F4] (STJA0005545)

SKU:
STJA0005545-100

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Host: Mouse
Applications: WB/IHC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Prion Protein is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 3F4
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC 1:100
ICC
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRNP
Gene ID: 5621
Uniprot ID: PRIO_HUMAN
Specificity: The antibody detects endogenous prion protein in human PC3 and A431 cell lines. In addition, the antibody strongly detects human recombinant Prion protein, and weakly detects bovine recombinant Prion protein.
Immunogen: Clone 3F4 was generated from a synthetic peptide taken from amino acids in the central region of human prion protein. This sequence is conserved in feline and hamster, but is less conserved in rat and mouse Prion protein.
Function Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.
Protein Name Major Prion Protein
Prp
Ascr
Prp27-30
Prp33-35c
Cd Antigen Cd230
Database Links
Reactome: R-HSA-419037
Reactome: R-HSA-9609523
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Targeted To Lipid Rafts Via Association With The Heparan Sulfate Chains Of Gpc1
Colocates
In The Presence Of Cu(2+)
To Vesicles In Para- And Perinuclear Regions
Where Both Proteins Undergo Internalization
Heparin Displaces Prnp From Lipid Rafts And Promotes Endocytosis
Alternative Antibody Names Anti-Major Prion Protein antibody
Anti-Prp antibody
Anti-Ascr antibody
Anti-Prp27-30 antibody
Anti-Prp33-35c antibody
Anti-Cd Antigen Cd230 antibody
Anti-PRNP antibody
Anti-ALTPRP antibody
Anti-PRIP antibody
Anti-PRP antibody

Information sourced from Uniprot.org

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