• Western blot analysis of lysates from wild type (WT) and Prion Protein knockout (KO) HeLa cells, using [KO Validated] Prion Protein Rabbit polyclonal antibody (STJ11100031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PRNP antibody (23-230) (STJ11100031)

SKU:
STJ11100031

€161.88 - €799.38
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRNP (23-230) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRNP
Gene ID: 5621
Uniprot ID: PRIO_HUMAN
Immunogen Region: 23-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 23-230 of human Prion Protein (NP_001073592.1).
Immunogen Sequence: KKRPKPGGWNTGGSRYPGQG SPGGNRYPPQGGGGWGQPHG GGWGQPHGGGWGQPHGGGWG QPHGGGWGQGGGTHSQWNKP SKPKTNMKHMAGAAAAGAVV GGLGGYMLGSAMSRPIIHFG SDYEDRYYRENMHRYPNQVY YRPMDEYSNQNNFVHDCVNI TIKQHTVTTTTKGENFTETD VKMMERVVEQMCITQYERES QAYYQRGS
Post Translational Modifications The glycosylation pattern (the amount of mono-, di- and non-glycosylated forms or glycoforms) seems to differ in normal and CJD prion.
Function Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as an agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu(2+) or Zn(2+) for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains.
Protein Name Major Prion Protein
Prp
Ascr
Prp27-30
Prp33-35c
Cd Antigen Cd230
Database Links
Reactome: R-HSA-419037
Reactome: R-HSA-9609523
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Golgi Apparatus
Targeted To Lipid Rafts Via Association With The Heparan Sulfate Chains Of Gpc1
Colocates
In The Presence Of Cu(2+)
To Vesicles In Para- And Perinuclear Regions
Where Both Proteins Undergo Internalization
Heparin Displaces Prnp From Lipid Rafts And Promotes Endocytosis
Alternative Antibody Names Anti-Major Prion Protein antibody
Anti-Prp antibody
Anti-Ascr antibody
Anti-Prp27-30 antibody
Anti-Prp33-35c antibody
Anti-Cd Antigen Cd230 antibody
Anti-PRNP antibody
Anti-ALTPRP antibody
Anti-PRIP antibody
Anti-PRP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance