![Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94075/375453/STJ115035_1__47849.1713142217.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
![Western blot analysis of lysates from mouse brain, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94075/375454/STJ115035_2__22315.1713142218.jpg?c=1 "Western blot analysis of lysates from mouse brain, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/94075/375453/STJ115035_1__47849.1713142217.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PRMT3 knockout (KO) 293T cells, using [KO Validated] PRMT3 Rabbit polyclonal antibody (STJ115035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
Anti-PRMT3 antibody (1-220) (STJ115035)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRMT3 (1-220) antibody for use in WB and ELISA in human and mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRMT3 |
| Gene ID | 10196 |
| Uniprot ID | ANM3_HUMAN |
| Immunogen Region | 1-220 |
| Immunogen Sequence | MCSLASGATGGRGAVENEED LPELSDSGDEAAWEDEDDAD LPHGKQQTPCLFCNRLFTSA EETFSHCKSEHQFNIDSMVH KHGLEFYGYIKLINFIRLKN PTVEYMNSIYNPVPWEKEEY LKPVLEDDLLLQFDVEDLYE PVSVPFSYPNGLSENTSVVE KLKHMEARALSAEAALARAR EDLQKMKQFAQDFVMHTDVR TCSSSTSVIADLQEDEDGVY |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRMT3 (NP_005779.1). |
Additional Info
| Function | Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases. Catalyzes the asymmetric arginine dimethylation at multiple sites in the Arg/Gly-rich region of small ribosomal subunit protein uS5/RPS2. Also appears to methylate other ribosomal proteins. May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. Contributes to methylation of histone H4 'Arg-3', a specific tag for epigenetic transcriptional activation. Mediates asymmetric arginine dimethylation of histone H4 'Arg-3' (H4R3me2a) in the promoter region of miRNA miR-3648, to promote its transcription and osteogenesis. |
| Protein Name | Protein Arginine N-Methyltransferase 3Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 |
| Database Links | Reactome: R-HSA-3214858Reactome: R-HSA-8876725 |
| Cellular Localisation | CytoplasmCytosolNucleusLocalized To The Nucleus By Znf200 |
| Alternative Antibody Names | Anti-Protein Arginine N-Methyltransferase 3 antibodyAnti-Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3 antibodyAnti-PRMT3 antibodyAnti-HRMT1L3 antibody |
Information sourced from Uniprot.org