Anti-PRKRA antibody (1-313) (STJ27370)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRKRA (1-313) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKRA |
| Gene ID | 8575 |
| Uniprot ID | PRKRA_HUMAN |
| Immunogen Region | 1-313 |
| Immunogen Sequence | MSQSRHRAEAPPLEREDSGT FSLGKMITAKPGKTPIQVLH EYGMKTKNIPVYECERSDVQ IHVPTFTFRVTVGDITCTGE GTSKKLAKHRAAEAAINILK ANASICFAVPDPLMPDPSKQ PKNQLNPIGSLQELAIHHGW RLPEYTLSQEGGPAHKREYT TICRLESFMETGKGASKKQA KRNAAEKFLAKFSNISPENH ISLTNVVGHSLGCTWHSLRN SPGEKINLLKRSLLSIPNT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human PRKRA/PACT (NP_003681.1). |
Additional Info
| Post Translational Modifications | Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress. |
| Function | Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner. |
| Protein Name | Interferon-Inducible Double-Stranded Rna-Dependent Protein Kinase Activator APkr-Associated Protein XPkr-Associating Protein XProtein Activator Of The Interferon-Induced Protein KinaseProtein Kinase - Interferon-Inducible Double-Stranded Rna-Dependent Activator |
| Database Links | Reactome: R-HSA-203927Reactome: R-HSA-426486Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmPerinuclear Region |
| Alternative Antibody Names | Anti-Interferon-Inducible Double-Stranded Rna-Dependent Protein Kinase Activator A antibodyAnti-Pkr-Associated Protein X antibodyAnti-Pkr-Associating Protein X antibodyAnti-Protein Activator Of The Interferon-Induced Protein Kinase antibodyAnti-Protein Kinase - Interferon-Inducible Double-Stranded Rna-Dependent Activator antibodyAnti-PRKRA antibodyAnti-PACT antibodyAnti-RAX antibodyAnti-HSD-14 antibodyAnti-HSD14 antibody |
Information sourced from Uniprot.org