• Western blot analysis of extracts of various cell lines, using PRKCSH antibody (STJ11102720) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PRKCSH antibody (429-528) [S0MR] (STJ11102720)

SKU:
STJ11102720

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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PRKCSH (429-528) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S0MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKCSH
Gene ID: 5589
Uniprot ID: GLU2B_HUMAN
Immunogen Region: 429-528
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 429-528 of human PRKCSH (P14314).
Immunogen Sequence: PFKLVSQKPKLGGSPTSLGT WGSWIGPDHDKFSAMKYEQG TGCWQGPNRSTTVRLLCGKE TMVTSTTEPSRCEYLMELMT PAACPEPPPEAPTEDDHDEL
Function Regulatory subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for efficient PKD1/Polycystin-1 biogenesis and trafficking to the plasma membrane of the primary cilia.
Protein Name Glucosidase 2 Subunit Beta
80k-H Protein
Glucosidase Ii Subunit Beta
Protein Kinase C Substrate 60.1 Kda Protein Heavy Chain
Pkcsh
Database Links Reactome: R-HSA-381426
Reactome: R-HSA-532668
Reactome: R-HSA-879415
Reactome: R-HSA-8957275
Reactome: R-HSA-901042
Reactome: R-HSA-9683686
Reactome: R-HSA-9694548
Cellular Localisation Endoplasmic Reticulum
Alternative Antibody Names Anti-Glucosidase 2 Subunit Beta antibody
Anti-80k-H Protein antibody
Anti-Glucosidase Ii Subunit Beta antibody
Anti-Protein Kinase C Substrate 60.1 Kda Protein Heavy Chain antibody
Anti-Pkcsh antibody
Anti-PRKCSH antibody
Anti-G19P1 antibody

Information sourced from Uniprot.org

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