![Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100335/386658/STJ25123_1__59754.1713155779.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
![Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/100335/386658/STJ25123_1__59754.1713155779.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] PKA RII Alpha (PRKAR2A) /PKR2 Rabbit polyclonal antibody (STJ25123) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.")
Anti-PRKAR2A antibody (1-404) (STJ25123)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRKAR2A (1-404) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKAR2A |
| Gene ID | 5576 |
| Uniprot ID | KAP2_HUMAN |
| Immunogen Region | 1-404 |
| Immunogen Sequence | MSHIQIPPGLTELLQGYTVE VLRQQPPDLVEFAVEYFTRL REARAPASVLPAATPRQSLG HPPPEPGPDRVADAKGDSES EEDEDLEVPVPSRFNRRVSV CAETYNPDEEEEDTDPRVIH PKTDEQRCRLQEACKDILLF KNLDQEQLSQVLDAMFERIV KADEHVIDQGDDGDNFYVIE RGTYDILVTKDNQTRSVGQY DNRGSFGELALMYNTPRAAT IVATSEGSLWGLDRVTFRR |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-404 of human PKA RII Alpha (PRKAR2A) /PKR2 (NP_004148.1). |
Additional Info
| Tissue Specificity | Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. |
| Post Translational Modifications | Phosphorylated by the activated catalytic chain. |
| Function | Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase. |
| Protein Name | Camp-Dependent Protein Kinase Type Ii-Alpha Regulatory Subunit |
| Database Links | Reactome: R-HSA-163615Reactome: R-HSA-164378Reactome: R-HSA-180024Reactome: R-HSA-381676Reactome: R-HSA-432040Reactome: R-HSA-442720Reactome: R-HSA-5610787Reactome: R-HSA-9010642Reactome: R-HSA-9634597Reactome: R-HSA-9660821Reactome: R-HSA-9664323Reactome: R-HSA-983231Reactome: R-HSA-9856530 |
| Cellular Localisation | CytoplasmCell MembraneColocalizes With Pja2 In The Cytoplasm And The Cell Membrane |
| Alternative Antibody Names | Anti-Camp-Dependent Protein Kinase Type Ii-Alpha Regulatory Subunit antibodyAnti-PRKAR2A antibodyAnti-PKR2 antibodyAnti-PRKAR2 antibody |
Information sourced from Uniprot.org