• STJ73243 (2µg/ml) staining of HeLa (A) and MCF7 (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-PRKACA antibody (Internal) (STJ73243)

SKU:
STJ73243-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PRKACA (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:8000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PRKACA
Gene ID: 5566
Uniprot ID: KAPCA_HUMAN
Immunogen Region: Internal
Accession Number: NP_002721.1; NP_997401.1; NP_001291278.1
Specificity: This antibody is expected to recognize all three reported isoforms (NP_002721.1; NP_997401.1; NP_001291278.1). Based on the choice of the immunizing peptide there is no expected cross-reactivity with the other subunits.
Immunogen Sequence: ESPAQNTAHLDQ
Post Translational Modifications Autophosphorylated. Phosphorylation is enhanced by vitamin K(2). Phosphorylated on threonine and serine residues. Phosphorylation on Thr-198 is required for full activity. Phosphorylated at Tyr-331 by activated receptor tyrosine kinases EGFR and PDGFR.this increases catalytic efficiency. Asn-3 is partially deaminated to Asp-3 giving rise to 2 major isoelectric variants, called CB and CA respectively. When myristoylated, Ser-11 is autophosphorylated probably in conjunction with deamidation of Asn-3.
Function Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA, SOX9 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in chondrogenesis by mediating phosphorylation of SOX9. Involved in the regulation of platelets in response to thrombin and collagen.maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Required for phosphorylation of GLI transcription factors which inhibits them and prevents transcriptional activation of Hedgehog signaling pathway target genes. GLI transcription factor phosphorylation is inhibited by interaction of PRKACA with SMO which sequesters PRKACA at the cell membrane. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis most probably through the regulation of OFD1 in ciliogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Phosphorylates HSF1.this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock. Acts as a negative regulator of mTORC1 by mediating phosphorylation of RPTOR. Isoform 2: Phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation.
Protein Name Camp-Dependent Protein Kinase Catalytic Subunit Alpha
Pka C-Alpha
Database Links Reactome: R-HSA-111931
Reactome: R-HSA-163358
Reactome: R-HSA-163560
Reactome: R-HSA-163615
Reactome: R-HSA-164378
Reactome: R-HSA-180024
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-381676
Reactome: R-HSA-392517
Reactome: R-HSA-422356
Reactome: R-HSA-432040
Reactome: R-HSA-4420097
Reactome: R-HSA-442720
Reactome: R-HSA-512988
Reactome: R-HSA-5578775
Reactome: R-HSA-5610780
Reactome: R-HSA-5610783
Reactome: R-HSA-5610785
Reactome: R-HSA-5610787
Reactome: R-HSA-5620912
Reactome: R-HSA-5621575
Reactome: R-HSA-5687128
Reactome: R-HSA-8853659
Reactome: R-HSA-8854518
Reactome: R-HSA-8963896
Reactome: R-HSA-9010642
Reactome: R-HSA-9022535
Reactome: R-HSA-9022692
Reactome: R-HSA-9634597
Reactome: R-HSA-9634600
Reactome: R-HSA-9660821
Reactome: R-HSA-9664323
Reactome: R-HSA-983231
Cellular Localisation Cytoplasm
Cell Membrane
Membrane
Lipid-Anchor
Nucleus
Mitochondrion
Translocates Into The Nucleus (Monomeric Catalytic Subunit)
The Inactive Holoenzyme Is Found In The Cytoplasm
Distributed Throughout The Cytoplasm In Meiotically Incompetent Oocytes
Associated To Mitochondrion As Meiotic Competence Is Acquired
Aggregates Around The Germinal Vesicles (Gv) At The Immature Gv Stage Oocytes
Colocalizes With Hsf1 In Nuclear Stress Bodies (Nsbs) Upon Heat Shock
Recruited To The Cell Membrane Through Interaction With Smo
Isoform 2: Cell Projection
Cilium
Flagellum
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Expressed In The Midpiece Region Of The Sperm Flagellum
Colocalizes With Mroh2b And Tcp11 On The Acrosome And Tail Regions In Round Spermatids And Spermatozoa Regardless Of The Capacitation Status Of The Sperm
Alternative Antibody Names Anti-Camp-Dependent Protein Kinase Catalytic Subunit Alpha antibody
Anti-Pka C-Alpha antibody
Anti-PRKACA antibody
Anti-PKACA antibody

Information sourced from Uniprot.org

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