• Immunofluorescence analysis of NIH/3T3 cells using PRKAB2 Rabbit polyclonal antibody (STJ29032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of Jurkat cells, using PRKAB2 antibody (STJ29032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using PRKAB2 antibody (STJ29032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKAB2 antibody (1-272) (STJ29032)

SKU:
STJ29032

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRKAB2 (1-272) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAB2
Gene ID: 5565
Uniprot ID: AAKB2_HUMAN
Immunogen Region: 1-272
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human PRKAB2 (NP_005390.1).
Immunogen Sequence: MGNTTSDRVSGERHGAKAAR SEGAGGHAPGKEHKIMVGST DDPSVFSLPDSKLPGDKEFV SWQQDLEDSVKPTQQARPTV IRWSEGGKEVFISGSFNNWS TKIPLIKSHNDFVAILDLPE GEHQYKFFVDGQWVHDPSEP VVTSQLGTINNLIHVKKSDF EVFDALKLDSMESSETSCRD LSSSPPGPYGQEMYAFRSEE RFKSPPILPPHLLQVILNKD TNISCDPALLPEPNHVMLN
Post Translational Modifications Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name 5'-Amp-Activated Protein Kinase Subunit Beta-2
Ampk Subunit Beta-2
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1632852
Reactome: R-HSA-163680
Reactome: R-HSA-200425
Reactome: R-HSA-2151209
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6804756
Reactome: R-HSA-9613354
Reactome: R-HSA-9619483
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Subunit Beta-2 antibody
Anti-Ampk Subunit Beta-2 antibody
Anti-PRKAB2 antibody

Information sourced from Uniprot.org

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