Anti-PRKAB2 antibody (1-272) (STJ29032)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRKAB2 (1-272) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKAB2 |
| Gene ID | 5565 |
| Uniprot ID | AAKB2_HUMAN |
| Immunogen Region | 1-272 |
| Immunogen Sequence | MGNTTSDRVSGERHGAKAAR SEGAGGHAPGKEHKIMVGST DDPSVFSLPDSKLPGDKEFV SWQQDLEDSVKPTQQARPTV IRWSEGGKEVFISGSFNNWS TKIPLIKSHNDFVAILDLPE GEHQYKFFVDGQWVHDPSEP VVTSQLGTINNLIHVKKSDF EVFDALKLDSMESSETSCRD LSSSPPGPYGQEMYAFRSEE RFKSPPILPPHLLQVILNKD TNISCDPALLPEPNHVMLN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-272 of human PRKAB2 (NP_005390.1). |
Additional Info
| Post Translational Modifications | Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1 and ULK2.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Beta-2Ampk Subunit Beta-2 |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-163680Reactome: R-HSA-200425Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Beta-2 antibodyAnti-Ampk Subunit Beta-2 antibodyAnti-PRKAB2 antibody |
Information sourced from Uniprot.org