STJ73638 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-PRKAB2 antibody (Internal) (STJ73638)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
STJ73638-100
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General Information

Short DescriptionGoat polyclonal antibody anti-PRKAB2 (Internal) is suitable for use in ELISA, Immunofluorescence, Immunohistochemistry and Flow Cytometry research applications.
ApplicationsPep-ELISA/IF/IHC/FC
HostGoat
ReactivityHuman/Mouse/Rat/Cow
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration0.5 mg/mL
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution RangePeptide ELISA: antibody detection limit dilution 1:8000.
IHC: Paraffin embedded Human Liver and Small Intestine. Recommended concentration: 3.75ยตg/ml.
IF: Strong expression of the protein seen in the nuclei of MCF7 and A431 cells. Recommended
Formulation0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Storage InstructionStore at-20ยฐC on receipt and minimise freeze-thaw cycles.

Target Information

Gene SymbolPRKAB2
Gene ID5565
Uniprot IDAAKB2_HUMAN
Accession NumberNP_005390.1
Immunogen RegionInternal
Immunogen SequencePYGQEMYAFRSE

Additional Info

Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name 5'-Amp-Activated Protein Kinase Subunit Beta-2
Ampk Subunit Beta-2
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1632852
Reactome: R-HSA-163680
Reactome: R-HSA-200425
Reactome: R-HSA-2151209
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6804756
Reactome: R-HSA-9613354
Reactome: R-HSA-9619483
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Subunit Beta-2 antibody
Anti-Ampk Subunit Beta-2 antibody
Anti-PRKAB2 antibody

Information sourced from Uniprot.org

Citations

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