• Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of MCF7 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded rat lung using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded mouse kidney using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of AMPKa1/AMPKa2 in paraffin-embedded human colon using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at dilution of 1:20 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ114591) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-PRKAA1/PRKAA2 antibody (13-559) (STJ114591)

SKU:
STJ114591

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAA2
PRKAA1
Gene ID: 5563
5562
Uniprot ID: AAPK2_HUMAN
AAPK1_HUMAN
Immunogen Region: 13-559
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 13-300 of human PRKAA1/PRKAA2 (NP_006242.5).
Immunogen Sequence: AEKQKHDGRVKIGHYILGDT LGVGTFGKVKVGKHELTGHK VAVKILNRQKIRSLDVVGKI RREIQNLKLFRHPHIIKLYQ VISTPSDIFMVMEYVSGGEL FDYICKNGRLDEKESRRLFQ QILSGVDYCHRHMVVHRDLK PENVLLDAHMNAKIADFGLS NMMSDGEFLRTSCGSPNYAA PEVISGRLYAGPEVDIWSSG VILYALLCGTLPFDDDHVPT LFKKICDGIFYTPQYLNPS
Tissue Specificity
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-2 antibody
Anti-Ampk Subunit Alpha-2 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-PRKAA2 antibody
Anti-AMPK antibody
Anti-AMPK2 antibody
Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-1 antibody
Anti-Ampk Subunit Alpha-1 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-Tau-Protein Kinase Prkaa1 antibody
Anti-PRKAA1 antibody
Anti-AMPK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance