• Western blot analysis of lysates from mouse spleen, using PRIM2 Rabbit polyclonal antibody (STJ113238) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of various lysates using PRIM2 Rabbit polyclonal antibody (STJ113238) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRIM2 antibody (1-170) (STJ113238)

SKU:
STJ113238

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRIM2 (1-170) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRIM2
Gene ID: 5558
Uniprot ID: PRI2_HUMAN
Immunogen Region: 1-170
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human PRIM2 (NP_000938.2).
Immunogen Sequence: MEFSGRKWRKLRLAGDQRNA SYPHCLQFYLQPPSENISLI EFENLAIDRVKLLKSVENLG VSYVKGTEQYQSKLESELRK LKFSYRENLEDEYEPRRRDH ISHFILRLAYCQSEELRRWF IQQEMDLLRFRFSILPKDKI QDFLKDSQLQFEAISDEEKT LREQEIVASS
Function Regulatory subunit of the DNA primase complex and component of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which play an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, an accessory subunit POLA2 and two primase subunits, the catalytic subunit PRIM1 and the regulatory subunit PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. In the primase complex, both subunits are necessary for the initial di-nucleotide formation, but the extension of the primer depends only on the catalytic subunit. Binds RNA:DNA duplex and coordinates the catalytic activities of PRIM1 and POLA2 during primase-to-polymerase switch.
Protein Name Dna Primase Large Subunit
Dna Primase 58 Kda Subunit
P58
Database Links Reactome: R-HSA-113501
Reactome: R-HSA-174411
Reactome: R-HSA-174430
Reactome: R-HSA-68952
Reactome: R-HSA-68962
Reactome: R-HSA-69091
Reactome: R-HSA-69166
Reactome: R-HSA-69183
Reactome: R-HSA-9710421
Alternative Antibody Names Anti-Dna Primase Large Subunit antibody
Anti-Dna Primase 58 Kda Subunit antibody
Anti-P58 antibody
Anti-PRIM2 antibody
Anti-PRIM2A antibody

Information sourced from Uniprot.org

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