• Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-spleen, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded human-kidney, antibody was diluted at 1:200
  • Western blot analysis of Hela cells using Perforin 1 Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-PRF1 antibody (451-500 aa) (STJ98623)

SKU:
STJ98623

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Perforin-1 (451-500 aa) is suitable for use in Western Blot, Immunohistochemistry and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:50-200
ELISA 1:10000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRF1
Gene ID: 5551
Uniprot ID: PERF_HUMAN
Immunogen Region: 451-500 aa
Specificity: Perforin 1 Polyclonal Antibody detects endogenous levels of Perforin 1
Immunogen: The antiserum was produced against synthesized peptide derived from the C-terminal region of human PRF1 at the amino acid range 451-500
Function Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu.
Protein Name Perforin-1
P1
Cytolysin
Lymphocyte Pore-Forming Protein
Pfp
Cellular Localisation Cytolytic Granule
Secreted
Cell Membrane
Multi-Pass Membrane Protein
Endosome Lumen
Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target Cell
Inserts Into The Cell Membrane Of Target Cells And Forms Pores
Membrane Insertion And Pore Formation Requires A Major Conformation Change
May Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early Endosomes
Alternative Antibody Names Anti-Perforin-1 antibody
Anti-P1 antibody
Anti-Cytolysin antibody
Anti-Lymphocyte Pore-Forming Protein antibody
Anti-Pfp antibody
Anti-PRF1 antibody
Anti-PFP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance