• Western blot analysis of extracts of various cell lines, using Peroxiredoxin 4 (PRDX4) (PRDX4) rabbit monoclonal antibody (STJ11102431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunofluorescence analysis of NIH-3T3 cells using Peroxiredoxin 4 (PRDX4) (PRDX4) Rabbit monoclonal antibody (STJ11102431) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat testis using Peroxiredoxin 4 (PRDX4) (PRDX4) rabbit monoclonal antibody (STJ11102431) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded human cervix cancer tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human placenta using Peroxiredoxin 4 (PRDX4) (PRDX4) rabbit monoclonal antibody (STJ11102431) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded human liver tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse brain using Peroxiredoxin 4 (PRDX4) (PRDX4) rabbit monoclonal antibody (STJ11102431) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded rat colon tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunofluorescence analysis of NIH-3T3 cells using Peroxiredoxin 4 (PRDX4) (PRDX4) rabbit monoclonal antibody (STJ11102431) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded human lung cancer tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded mouse testis tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded human colon carcinoma tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Peroxiredoxin 4 (PRDX4) in paraffin-embedded human thyroid cancer tissue using Peroxiredoxin 4 (PRDX4) Rabbit monoclonal antibody (STJ11102431) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using Peroxiredoxin 4 (PRDX4) (PRDX4) Rabbit monoclonal antibody (STJ11102431) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PRDX4 antibody (1-100) [S1MR] (STJ11102431)

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STJ11102431

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Peroxiredoxin 4 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDX4
Gene ID: 10549
Uniprot ID: PRDX4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Peroxiredoxin 4 (PRDX4) (PRDX4) (Q13162).
Immunogen Sequence: MEALPLLAATTPDHGRHRRL LLLPLLLFLLPAGAVQGWET EERPRTREEECHFYAGGQVY PGEASRVSVADHSLHLSKAK ISKPAPYWEGTAVIDGEFKE
Post Translational Modifications The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.
Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Protein Name Peroxiredoxin-4
Antioxidant Enzyme Aoe372
Aoe37-2
Peroxiredoxin Iv
Prx-Iv
Thioredoxin Peroxidase Ao372
Thioredoxin-Dependent Peroxide Reductase A0372
Thioredoxin-Dependent Peroxiredoxin 4
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Cotranslationally Translocated To And Retained Within The Endoplasmic Reticulum
A Small Fraction Of The Protein Is Cytoplasmic
Alternative Antibody Names Anti-Peroxiredoxin-4 antibody
Anti-Antioxidant Enzyme Aoe372 antibody
Anti-Aoe37-2 antibody
Anti-Peroxiredoxin Iv antibody
Anti-Prx-Iv antibody
Anti-Thioredoxin Peroxidase Ao372 antibody
Anti-Thioredoxin-Dependent Peroxide Reductase A0372 antibody
Anti-Thioredoxin-Dependent Peroxiredoxin 4 antibody
Anti-PRDX4 antibody

Information sourced from Uniprot.org

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