Western blot analysis of extracts from normal (control) and Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4) ) knockout (KO) 293T cells, using Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4) ) antibody (STJ25106) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PRDX4 antibody (38-271) (STJ25106)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ25106
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General Information

Short DescriptionRabbit polyclonal PRDX4 (38-271) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolPRDX4
Gene ID10549
Uniprot IDPRDX4_HUMAN
Immunogen Region38-271
Immunogen SequenceWETEERPRTREEECHFYAGG QVYPGEASRVSVADHSLHLS KAKISKPAPYWEGTAVIDGE FKELKLTDYRGKYLVFFFYP LDFTFVCPTEIIAFGDRLEE FRSINTEVVACSVDSQFTHL AWINTPRRQGGLGPIRIPLL SDLTHQISKDYGVYLEDSGH TLRGLFIIDDKGILRQITLN DLPVGRSVDETLRLVQAFQY TDKHGEVCPAGWKPGSETII PDPAGKLKYFDKLN
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 38-271 of human Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4) ) (NP_006397.1).

Additional Info

Post Translational Modifications The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond.
Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Protein Name Peroxiredoxin-4
Antioxidant Enzyme Aoe372
Aoe37-2
Peroxiredoxin Iv
Prx-Iv
Thioredoxin Peroxidase Ao372
Thioredoxin-Dependent Peroxide Reductase A0372
Thioredoxin-Dependent Peroxiredoxin 4
Database Links Reactome: R-HSA-6798695
Cellular Localisation Cytoplasm
Endoplasmic Reticulum
Cotranslationally Translocated To And Retained Within The Endoplasmic Reticulum
A Small Fraction Of The Protein Is Cytoplasmic
Alternative Antibody Names Anti-Peroxiredoxin-4 antibody
Anti-Antioxidant Enzyme Aoe372 antibody
Anti-Aoe37-2 antibody
Anti-Peroxiredoxin Iv antibody
Anti-Prx-Iv antibody
Anti-Thioredoxin Peroxidase Ao372 antibody
Anti-Thioredoxin-Dependent Peroxide Reductase A0372 antibody
Anti-Thioredoxin-Dependent Peroxiredoxin 4 antibody
Anti-PRDX4 antibody

Information sourced from Uniprot.org

Citations

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