| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Thioredoxin-Dependent Peroxide Reductase-Mitochondrial (63-256) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:5000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRDX3 |
| Gene ID: |
10935 |
| Uniprot ID: |
PRDX3_HUMAN |
| Immunogen Region: |
63-256 |
| Immunogen: |
Recombinant Protein corresponding to a sequence within amino acids 63-256 of human Peroxiredoxin 3 (PRDX3) (NP_006784.1). |
| Immunogen Sequence: |
PAVTQHAPYFKGTAVVNGEF KDLSLDDFKGKYLVLFFYPL DFTFVCPTEIVAFSDKANEF HDVNCEVVAVSVDSHFSHLA WINTPRKNGGLGHMNIALLS DLTKQISRDYGVLLEGSGLA LRGLFIIDPNGVIKHLSVND LPVGRSVEETLRLVKAFQYV ETHGEVCPANWTPDSPTIKP SPAASKEYFQKVNQ |
| Post Translational Modifications | Phosphorylated by LRRK2.phosphorylation reduces perodixase activity. The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) and sulphonic acid (C(P)-SO3H) instead of its condensation to a disulfide bond. S-palmitoylated. |
| Function | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides. Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol. Required for the maintenance of physical strength. |
| Protein Name | Thioredoxin-Dependent Peroxide Reductase - MitochondrialAntioxidant Protein 1Aop-1Hbc189Peroxiredoxin IiiPrx-IiiPeroxiredoxin-3Protein Mer5 HomologThioredoxin-Dependent Peroxiredoxin 3 |
| Database Links | Reactome: R-HSA-3299685 |
| Cellular Localisation | MitochondrionCytoplasmEarly EndosomeLocalizes To Early Endosomes In A Rps6kc1-Dependent Manner |
| Alternative Antibody Names | Anti-Thioredoxin-Dependent Peroxide Reductase - Mitochondrial antibodyAnti-Antioxidant Protein 1 antibodyAnti-Aop-1 antibodyAnti-Hbc189 antibodyAnti-Peroxiredoxin Iii antibodyAnti-Prx-Iii antibodyAnti-Peroxiredoxin-3 antibodyAnti-Protein Mer5 Homolog antibodyAnti-Thioredoxin-Dependent Peroxiredoxin 3 antibodyAnti-PRDX3 antibodyAnti-AOP1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3 (PRDX3) ) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 (PRDX3) Rabbit polyclonal antibody (STJ119995) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/98780/382829/STJ119995_1__29019.1713151331.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3 (PRDX3) ) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 (PRDX3) Rabbit polyclonal antibody (STJ119995) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
![Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3 (PRDX3) ) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 (PRDX3) Rabbit polyclonal antibody (STJ11100034) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89106/357562/STJ11100034_1__50139.1713121688.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Peroxiredoxin 3 (Peroxiredoxin 3 (PRDX3) ) knockout (KO) HeLa cells, using [KO Validated] Peroxiredoxin 3 (PRDX3) Rabbit polyclonal antibody (STJ11100034) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")
